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PMID: 4340157 Published · ppublish English Journal Article

Selective inhibition of nuclear DNA synthesis by 9- -D-arabinofuranosyl adenine in rat cells transformed by Rous sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 7 ·1972-07-00 ·Pages 1753-7

Shipman C, Smith SH, Drach JC

Abstract

The drug, 9-beta-D-arabinofuranosyl adenine, selectively inhibits the synthesis of nuclear DNA without affecting extrachromosomal DNA synthesis in rat cells transformed by Rous sarcoma virus (B-mix K-44/6). The inhibition was linear with respect to drug concentration over the range of 37-600 muM. Mitosis and total synthesis of DNA per cell were also depressed. DNA synthesis was determined by measurement of [(3)H]thymidine incorporation into DNA. Covalently-closed circular DNA was extracted by the Hirt procedure and separated from residual chromosomal DNA by buoyant density gradient ultracentrifugation in CsCl-propidium diiodide. On the basis of buoyant density and sedimentation velocity centrifugation, the covalently-closed circular DNA formed in the presence of the drug was indistinguishable from that formed in its absence.

MeSH Terms
Adenine/pharmacology Animals Arabinose/pharmacology Avian Sarcoma Viruses Cell Line Cell Nucleus/drug effects,metabolism Cell Transformation, Neoplastic Cells, Cultured/drug effects,metabolism Centrifugation, Density Gradient DNA/biosynthesis Embryo, Mammalian/drug effects,metabolism Iodides Mitosis/drug effects Nucleosides/pharmacology Phenanthridines Rats Thymidine/metabolism Tritium
Chemicals
Iodides Nucleosides Phenanthridines Tritium DNA Arabinose Adenine Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shipman C
Smith S H
Drach J C
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.7.1753
Published
1972-07-00
Pages
1753-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426795
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.7.1753 PMC Full Text View journal details
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