Analysis of the fusion of XC cells induced by homogenates of murine leukemia virus-infected cells and by purified murine leukemia virus.

Journal of virology ·Vol. 7 ·No. 6 ·1971-06-00 ·Pages 753-8

Johnson GS, Friedman RM, Pastan I

Abstract

The fusion of XC cells induced by murine leukemia virus (MuLV)-infected cells is also induced by homogenates prepared from the infected cells and by purified MuLV. The fusion-inducing factor appears to contain a heat-labile lipoprotein. No synthesis of specific macromolecules by the XC cells is necessary to obtain fusion. The results suggest that specific components of the viral particle are the activators for the fusion process and they may also be present in the membranes of infected cells.

MeSH Terms

Animals Cell Biology Cell Fusion Cell Line Cell Membrane/microbiology Centrifugation, Density Gradient Centrifugation, Zonal Culture Media Culture Techniques Cytopathogenic Effect, Viral Deoxyribonucleases Hot Temperature Lipase Lipoproteins Mice Microscopy, Electron Moloney murine leukemia virus/isolation & purification,pathogenicity Neuraminidase Rats Ribonucleases Sarcoma, Avian Sucrose Trypsin

Chemicals

Culture Media Lipoproteins Sucrose Deoxyribonucleases Ribonucleases Lipase Neuraminidase Trypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson G S

Friedman R M

Pastan I

References (16)

16 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.6.753-758.1971

Published

1971-06-00

Pages

753-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356194

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.6.753-758.1971 PMC Full Text View journal details

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Analysis of the fusion of XC cells induced by homogenates of murine leukemia virus-infected cells and by purified murine leukemia virus.

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