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PMID: 4323307 Published · ppublish English Journal Article

Ionic mechanism of post-tetanic potentiation at the neuromuscular junction of the frog.

The Journal of physiology ·Vol. 212 ·No. 2 ·1971-01-00 ·Pages 431-46

Weinreich D

Abstract

1. Transmitter release at the frog neuromuscular junction was studied after sodium influx in nerve and muscle was abolished by tetrodotoxin (TTX).2. In the presence of TTX, transmitter release evoked by electrotonic depolarization of the nerve terminal was potentiated following presynaptic stimulation by a train of depolarizing pulses.3. Post-tetanic potentiation (PTP) in the presence of TTX appeared no different from that observed in control (TTX-free) muscles. The magnitude as well as the time course of PTP was dependent on the number of tetanic stimuli and on temperature of the medium when sodium influx was inhibited by TTX.4. When external sodium was replaced by an isotonic calcium chloride solution PTP was still present. Ionophoretic application of calcium during tetanic nerve stimulation and increase in the intensity of the depolarizing pulse of the train, which presumably enhances calcium movements into nerve endings, caused a large increase in the duration of PTP.5. It is concluded that PTP does not require sodium but depends on movement of calcium from the external medium into the nerve terminal.

MeSH Terms
Action Potentials/drug effects Animals Anura Calcium/metabolism Calcium Chloride/pharmacology Electric Stimulation Electrophysiology Iontophoresis Isotonic Solutions/pharmacology Motor Neurons/drug effects Neuromuscular Junction/metabolism,physiology Sodium/metabolism Synaptic Transmission/drug effects Temperature Tetrodotoxin/pharmacology Time Factors
Chemicals
Isotonic Solutions Tetrodotoxin Sodium Calcium Chloride Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Weinreich D
References (43)
43 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1971.sp009333
Published
1971-01-00
Pages
431-46
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1395675
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1971.sp009333 PMC Full Text View journal details
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