Adenovirus type 2-simian virus 40 hybrid population: evidence for a hybrid deoxyribonucleic acid molecule and the absence of adenovirus-encapsidated circular simian virus 40 deoxyribonucleic acid.

Journal of virology ·Vol. 6 ·No. 6 ·1970-12-00 ·Pages 788-94

Crumpacker CS, Levin MJ, Wiese WH, Rowe WP, Lewis AM

Abstract

The deoxyribonucleic acid (DNA) from the adenovirus-encapsidated particles of the adenovirus type 2 (Ad2)-simian virus 40 (SV40) hybrid population plaque variant (Ad2(++) HEY), known to yield SV40 virus with high efficiency, was studied by equilibrium density centrifugation followed by ribonucleic acid-DNA hybridization employing virus-specific complementary ribonucleic acids synthesized in vitro. These techniques establish linkage between the Ad2 and SV40 components in the adenovirus-encapsidated particles of this population. The linkage is alkali-resistant and presumably covalent; thus, the Ad2 DNA and SV40 DNA are present in a hybrid molecule. Velocity centrifugation studies in alkaline sucrose gradients eliminated the possibility that supercoiled circular SV40 DNA is present in the adenovirus capsids. The DNA obtained from the adenovirus-encapsidated particles of the Ad2(++) HEY population appears to consist of nonhybrid Ad2 DNA and Ad2-SV40 hybrid DNA molecules.

MeSH Terms

Adenoviridae/analysis,growth & development Animals Cell Line Centrifugation, Density Gradient Cesium Chlorides DNA, Viral/analysis,isolation & purification Genetic Variation Genetics, Microbial Haplorhini Humans Hybridization, Genetic Kidney Molecular Biology Nucleic Acid Denaturation Phosphorus Isotopes RNA, Viral Simian virus 40/analysis,growth & development Sucrose Thymidine Tritium

Chemicals

Chlorides DNA, Viral Phosphorus Isotopes RNA, Viral Tritium Cesium Sucrose Thymidine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Crumpacker C S

Levin M J

Wiese W H

Rowe W P

Lewis A M

References (20)

20 references, click to expand

BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY.
J Immunol. 1963 Sep;91:416-24 PMID: 14071033
Isolation of two plaque variants from the adenovirus type 2-simian virus 40 hybrid population which differ in their efficiency in yielding simian virus 40.
J Virol. 1970 Apr;5(4):413-20 PMID: 4318083
Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome.
Proc Natl Acad Sci U S A. 1966 Mar;55(3):526-31 PMID: 4287488
The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form.
J Mol Biol. 1965 Dec;14(2):327-47 PMID: 5880854
Virus-specific nucleic acids in SV40-exposed hamster embryo cell lines: correlation with S and T antigens.
Proc Natl Acad Sci U S A. 1969 Feb;62(2):589-96 PMID: 4307716
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus.
Proc Soc Exp Biol Med. 1966 May;122(1):214-8 PMID: 4287655
THE PURIFICATION OF SIMIAN VIRUS 40.
Virology. 1964 Nov;24:381-7 PMID: 14227039
THE NUCLEIC ACID OF SIMIAN VIRUS 40.
Virology. 1964 Nov;24:388-92 PMID: 14227040
Virus-specific deoxyribonucleic acid in simian virus 40-exposed hamster cells: correlation with S and T antigens.
J Virol. 1970 Aug;6(2):199-207 PMID: 4322872
VIRAL STUDIES OF SV40 TUMORIGENESIS IN HAMSTERS.
J Natl Cancer Inst. 1964 Jan;32:253-65 PMID: 14114971
Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.
Proc Natl Acad Sci U S A. 1962 Jan 15;48:81-94 PMID: 13877961
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
Equilibrium density gradient studies on simian virus 40-yielding variants of the adenovirus type 2-simian virus 40 hybrid population.
J Virol. 1970 Apr;5(4):421-6 PMID: 4318084
RNA production in adenovirus-infected KB cells.
Virology. 1965 Dec;27(4):571-9 PMID: 5855573
Similarity of DNAs isolated from tumor-inducing viruses of human and animal origin.
Proc Natl Acad Sci U S A. 1963 Jul;50:44-6 PMID: 13950094
EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.
Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581-8 PMID: 16590059
Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles.
Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509-15 PMID: 4291405
Production of highly labeled adenoviruses.
Appl Microbiol. 1968 Aug;16(8):1245-50 PMID: 5675512
Tissue cultures and mycoplasmas.
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.6.6.788-794.1970

Published

1970-12-00

Pages

788-94

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC376196

Subset

External Links

PubMed source DOI: 10.1128/JVI.6.6.788-794.1970 PMC Full Text View journal details

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Adenovirus type 2-simian virus 40 hybrid population: evidence for a hybrid deoxyribonucleic acid molecule and the absence of adenovirus-encapsidated circular simian virus 40 deoxyribonucleic acid.

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