Production of highly labeled adenoviruses.

Applied microbiology ·Vol. 16 ·No. 8 ·1968-08-00 ·Pages 1245-50

Burnett JP, Summers AO, Harrington JA, Dwyer AC

Abstract

A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.

MeSH Terms

Adenoviridae/drug effects,growth & development,isolation & purification,metabolism Centrifugation, Density Gradient Culture Techniques DNA, Viral/metabolism Hydrolases/pharmacology Methotrexate/pharmacology Microscopy, Electron Thymidine/metabolism Tritium Virus Cultivation

Chemicals

DNA, Viral Tritium Hydrolases Thymidine Methotrexate

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Burnett J P

Summers A O

Harrington J A

Dwyer A C

References (12)

12 references, click to expand

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Proc Natl Acad Sci U S A. 1967 May;57(5):1286-93 PMID: 4292150
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Virology. 1963 May;20:199-207 PMID: 13950097
Prevention of toxicity of amethopterin for sarcoma-180 cells in tissue culture.
Science. 1957 Aug 9;126(3267):255 PMID: 13454808
THE MECHANISM OF ACTION OF THE FOLATE ANTAGONISTS IN MAN.
Cancer Res. 1963 Sep;23:1286-306 PMID: 14070383
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J Histochem Cytochem. 1956 Jan;4(1):1-10 PMID: 13295564
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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Amino acid metabolism in mammalian cell cultures.
Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
Antigens and structure of the adenovirus.
J Mol Biol. 1965 Aug;13(1):13-20 PMID: 4955344
Thymidine kinase activity in synchronized HeLa cell cultures.
Biochem Biophys Res Commun. 1965 Aug 16;20(4):535-8 PMID: 5860172
Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid.
J Virol. 1967 Oct;1(5):851-67 PMID: 5621482
BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION. VII. HOMOLOGY BETWEEN DNA'S OF TUMORIGENIC AND NONTUMORIGENIC HUMAN ADENOVIRUSES.
Proc Natl Acad Sci U S A. 1964 Oct;52:1053-9 PMID: 14224385

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.16.8.1245-1250.1968

Published

1968-08-00

Pages

1245-50

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC547627

Subset

External Links

PubMed source DOI: 10.1128/am.16.8.1245-1250.1968 PMC Full Text

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Production of highly labeled adenoviruses.

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