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PMID: 4201264 Published · ppublish English Journal Article

Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy.

The Journal of clinical investigation ·Vol. 52 ·No. 11 ·1973-11-00 ·Pages 2737-44

Hoyer LW, De los Santos RP, Hoyer JR

Abstract

The tissue localization of antihemophilic factor (AHF, Factor VIII) has been determined by immunofluorescent studies using monospecific rabbit antibody to human AHF. Specific staining demonstrating AHF antigens has been identified in endothelial cells of a wide range of human tissues. The staining pattern was observed in endothelial cells of arteries, capillaries, and veins as well as the cells lining hepatic and splenic sinusoids. Specific fluorescence was limited to these endothelial cells in sections of kidney, liver, spleen, lymph node, cardiac and smooth muscle, thyroid, umbilical cord, and skin. Absorption studies established that the staining was specific for cells in which there were proteins that had AHF antigens. The demonstration of fluorescence within the cytoplasm of endothelial cells suggests that these cells synthesize proteins that have AHF antigens.

MeSH Terms
Animals Antigens/analysis Aorta/immunology Endothelium/immunology Factor VIII Fluorescent Antibody Technique Goats/immunology Humans Immune Sera Immunodiffusion Immunoelectrophoresis Kidney/immunology Liver/immunology Lymph Nodes/immunology Muscles/immunology Myocardium/immunology Portal Vein/immunology Rabbits/immunology Skin/immunology Spleen/immunology Thyroid Gland/immunology Umbilicus/immunology
Chemicals
Antigens Immune Sera Factor VIII
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoyer L W
De los Santos R P
Hoyer J R
References (36)
36 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107469
Published
1973-11-00
Pages
2737-44
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC302541
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI107469 PMC Full Text View journal details
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