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PMID: 4634046 Published · ppublish English Journal Article

Tissue factor activity in lymphocyte cultures from normal individuals and patients with hemophilia A.

The Journal of clinical investigation ·Vol. 52 ·No. 6 ·1973-06-00 ·Pages 1427-34

Rickles FR, Hardin JA, Pitlick FA, Hoyer LW, Conrad ME

Abstract

The procoagulant material of lymphocytes has been characterized as tissue factor. Lymphocytes stimulated with phytohemagglutinin or the purified protein derivative of the tubercle bacillus developed procoagulant activity with incubation in tissue culture. While this material corrected the prolonged clotting time of factor VIII (AHF) deficient plasma, we have shown, utilizing a sensitive radioimmunoassay, that no AHF antigen was present in the cell cultures. Further, we have demonstrated this material to be tissue factor by coagulation techniques and immunological cross-reactivity. The published data regarding factor VIII synthesis is reviewed in light of these observations and comments are made regarding the role of the lymphocyte procoagulant.

MeSH Terms
Animals Antigens/analysis Blood Coagulation Factors/analysis Cells, Cultured Centrifugation, Density Gradient Cross Reactions Factor VIII/analysis Hemophilia A/immunology Humans Iodine Isotopes Lectins/pharmacology Leukocytes/drug effects,immunology Lymphocytes/drug effects,immunology Methods Micropore Filters Mycobacterium tuberculosis Placenta/immunology Proteins/pharmacology Rabbits/immunology Radioimmunoassay Time Factors Tritium
Chemicals
Antigens Blood Coagulation Factors Iodine Isotopes Lectins Proteins Tritium Factor VIII
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rickles F R
Hardin J A
Pitlick F A
Hoyer L W
Conrad M E
References (44)
44 references, click to expand
  1. Antihaemophilic globulin (AHG) in multiple myeloma and macroglobulinaemia.
    Br J Haematol. 1968 Feb;14(2):205-14 PMID: 4170417
  2. Antihemophilic factor release by perfused liver and spleen: relationship to hemophilia.
    Science. 1967 Nov;158(3804):1060-1 PMID: 6058346
  3. Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen.
    J Lab Clin Med. 1972 Dec;80(6):822-33 PMID: 4634503
  4. Immunologic differentiation of classic hemophilia (factor 8 deficiency) and von Willebrand's dissase, with observations on combined deficiencies of antihemophilic factor and proaccelerin (factor V) and on an acquired circulating anticoagulant against antihemophilic factor.
    J Clin Invest. 1971 Jan;50(1):244-54 PMID: 5543879
  5. Cell-culture synthesis of a factor VIII-like activity.
    Mayo Clin Proc. 1969 Nov;44(11):784-92 PMID: 4981692
  6. The role of protein synthesis on the generation of tissue factor activity by leukocytes.
    Proc Soc Exp Biol Med. 1972 Apr;139(4):1276-9 PMID: 5023325
  7. Attempted spleen transplant in classical hemophilia.
    Transplantation. 1969 Jan;7(1):73-5 PMID: 5762196
  8. Transplantation of the spleen.
    Ann Intern Med. 1968 Mar;68(3):700-4 PMID: 4868461
  9. The partial thromboplastin time with kaolin. A simple screening test for first stage plasma clotting factor deficiencies.
    Am J Clin Pathol. 1961 Sep;36:212-9 PMID: 13738153
  10. Endotoxin-induced intravascular clotting: the need for granulocytes.
    Thromb Diath Haemorrh. 1968 Dec 31;20(3):430-43 PMID: 5751635
  11. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.
    Scand J Clin Lab Invest Suppl. 1968;97:77-89 PMID: 4179068
  12. Hemophilia: role of organ homografts.
    Science. 1969 Jan 10;163(3863):188-90 PMID: 4883723
  13. FACTORS AFFECTING THE DYE EXCLUSION TEST FOR CELL VIABILITY.
    Exp Cell Res. 1964 Jun;35:9-13 PMID: 14190667
  14. Characterization of the coagulant activity of cultured human fibroblasts.
    Blood. 1971 Jan;37(1):47-51 PMID: 5539131
  15. Release of factor 8 (antihaemophilic factor) from perfused organs and tissues.
    Nature. 1967 Mar 18;213(5081):1146-7 PMID: 6040180
  16. Effect of antihemophilic factor on one-stage clotting tests; a presumptive test for hemophilia and a simple one-stage antihemophilic factor assy procedure.
    J Lab Clin Med. 1953 Apr;41(4):637-47 PMID: 13045017
  17. The role of the leukocyte in the generalized Shwartzman reaction.
    Lab Invest. 1967 Apr;16(4):511-5 PMID: 5337163
  18. Transplantation of the spleen: experimental cure of hemophilia.
    Surgery. 1968 Jul;64(1):1-14 PMID: 4173158
  19. [Modifications of blood coagulation by means of leukocytes].
    Klin Wochenschr. 1951 Aug 1;29(29-30):510-2 PMID: 14862102
  20. Stimulation of human leukocyte thromboplastic activity by endotoxin.
    Proc Soc Exp Biol Med. 1971 Oct;138(1):145-8 PMID: 4942184
  21. The thromboplastic activity of white blood cells.
    Blut. 1968 Aug;17(5):276-8 PMID: 5664357
  22. Thromboplastic activity of leukemic white cells.
    Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):763-5 PMID: 13204348
  23. Role of the lymphocyte in antihemophilic globulin production: a rise in antihemophilic globulin levels in a hemophilic subject with acute lymphoblastic leukemia.
    J Lab Clin Med. 1971 Jul;78(1):122-9 PMID: 5285069
  24. Immunologic studies of antihemophilic factor (AHF, factor VIII). 3. Comparative binding properties of human and rabbit anti-AHF.
    Blood. 1972 Apr;39(4):481-9 PMID: 4622205
  25. The phospholipid requirement of tissue factor in blood coagulation.
    J Clin Invest. 1968 Jan;47(1):72-80 PMID: 16695947
  26. Ethionine-induced depression of plasma antihemophilic globulin in the rat.
    Proc Soc Exp Biol Med. 1954 Oct;87(1):54-7 PMID: 13224675
  27. The blood clotting properties of rabbit peritoneal leukocytes in vitro.
    Thromb Diath Haemorrh. 1967 Feb 28;17(1-2):222-36 PMID: 5584955
  28. Coagulant activity of leukocytes. Tissue factor activity.
    J Clin Invest. 1972 Feb;51(2):307-13 PMID: 4333021
  29. Antihemophilic factor activity isolated from kidneys of normal and hemophilic dogs.
    Am J Physiol. 1971 Apr;220(4):1020-4 PMID: 4101480
  30. Independent stimulators regulating the production of coagulation factors VIII and IX in perfused spleens.
    J Lab Clin Med. 1972 May;79(5):770-7 PMID: 4537012
  31. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report.
    Transplantation. 1971 Mar;11(3):268-73 PMID: 5558562
  32. Synthesis of clotting factors by the isolated perfused rat liver.
    J Clin Invest. 1966 May;45(5):690-701 PMID: 5935358
  33. Synthesis of antihemophilic factor (Factor VIII) by leukocytes: preliminary report.
    Mayo Clin Proc. 1968 Sep;43(9):617-9 PMID: 5751674
  34. Storage, release, and synthesis of coagulation factors in isolated perfused organs.
    Am J Physiol. 1969 Sep;217(3):879-83 PMID: 5807715
  35. Development of procoagulant (tissue thromboplastin) activity in cultured leukocytes.
    J Lab Clin Med. 1972 May;79(5):778-87 PMID: 5018741
  36. Hepatic influence on splenic synthesis and release of coagulation activities.
    Science. 1969 Nov 14;166(3907):882-3 PMID: 5307348
  37. The physiologic basis for therapy of classic hemophilia (factor 8 deficiency) and related disorders. Combined clinical staff conference at the National Institutes of Health.
    Ann Intern Med. 1967 Oct;67(4):856-82 PMID: 5299081
  38. Plasma factor VIII synthesis and control as revealed by canine organ transplantation.
    Am J Physiol. 1971 May;220(5):1147-54 PMID: 4930132
  39. Tissue factor (thromboplastin): localization to plasma membranes by peroxidase-conjugated antibodies.
    Science. 1972 Feb 18;175(4023):766-8 PMID: 5057816
  40. Purification and characterization of the protein component of tissue factor.
    Biochemistry. 1970 Dec 22;9(26):5100-5 PMID: 4098334
  41. The Stuart-Prower factor assay and its clinical significance.
    Thromb Diath Haemorrh. 1958 May 1;2(1-2):24-38 PMID: 13581015
  42. Role of leukocytes in blood coagulation and the generalized Shwartzman reaction.
    Nat New Biol. 1971 Aug 25;232(34):247-8 PMID: 5286196
  43. Procoagulant synthesis by cultured fibroblasts triggered by cell adhesion.
    Nature. 1971 Jul 30;232(5309):338-9 PMID: 5094839
  44. The role of the splenic macrophage in antihemophilic factor (factor VIII) synthesis.
    Arch Surg. 1971 Sep;103(3):398-401 PMID: 5569927
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107316
Published
1973-06-00
Pages
1427-34
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC302407
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI107316 PMC Full Text View journal details
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