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PMID: 4198517 Published · ppublish English Journal Article

The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum.

The Biochemical journal ·Vol. 133 ·No. 1 ·1973-05-00 ·Pages 37-47

Every D, Ashworth JM

Abstract

The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.

MeSH Terms
Acetamides Chromatography, DEAE-Cellulose Chromatography, Gel Concanavalin A Drug Stability Electrophoresis, Polyacrylamide Gel Electrophoresis, Starch Gel Glucosidases/isolation & purification,metabolism Glycoside Hydrolases/isolation & purification,metabolism Hexosaminidases/isolation & purification,metabolism Hydrogen-Ion Concentration Immunodiffusion Isoenzymes Kinetics Mannose Molecular Weight Myxomycetes/enzymology Nitrophenols Spectrophotometry Temperature Time Factors
Chemicals
Acetamides Isoenzymes Nitrophenols Concanavalin A Glucosidases Glycoside Hydrolases Hexosaminidases Mannose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Every D
Ashworth J M
References (39)
39 references, click to expand
  1. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
    Biochem J. 1965 Sep;96(3):595-606 PMID: 5862401
  2. Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.
    Biochem J. 1967 Feb;102(2):525-32 PMID: 6029611
  3. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
    Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
  4. Glycosidases of Phaseolus vulgaris. II. Isolation and general properties.
    J Biol Chem. 1968 Jan 10;243(1):103-11 PMID: 5639691
  5. N-Acetyl-beta-glucosaminidases in human spleen.
    Biochem J. 1968 Apr;107(3):321-7 PMID: 5650361
  6. [Isolation, purification and some physicochemical characteristics of 2 beta-hexosidases of the digestive juice of Helix pomatia].
    Eur J Biochem. 1968 Apr 3;4(2):240-6 PMID: 5655500
  7. Alpha-mannosidase and beta-mannosidase from the liver of Tubo cortunus: purfication, properties and application of carbohydrate research.
    J Biochem. 1967 Dec;62(6):700-9 PMID: 4968617
  8. N-acetylhexosaminidases from a gastropod, Turbo cornutus.
    J Biochem. 1968 Oct;64(4):521-31 PMID: 4974274
  9. Polysaccharides in vegetative and aggregation-competent amoebae of Dictyostelium discoideum. 2. Purification and characterization of amoeba-degraded bacterial polysaccharides.
    Eur J Biochem. 1969 Jan;7(2):239-46 PMID: 4885465
  10. Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum.
    J Bacteriol. 1969 Mar;97(3):1149-54 PMID: 5813341
  11. Glycosidases of Aspergillus niger. I. Purification and characterization of alpha- and beta-galactosidases and beta-N-acetylglucosaminidase.
    J Biol Chem. 1969 Jun 10;244(11):2970-8 PMID: 5772469
  12. Isolation and characterization of beta-galactosidase from Saccharomyces lactis.
    Biochim Biophys Acta. 1968 Oct 8;167(2):373-7 PMID: 5729952
  13. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  14. Acid alpha-D-glucosidase glucohydrolase from cattle liver.
    J Biol Chem. 1969 Sep 10;244(17):4735-42 PMID: 5808513
  15. Purification and properties of a beta-hexosidase from Sporobolomyces singularis.
    Can J Biochem. 1969 Nov;47(11):1021-5 PMID: 5389663
  16. Isozymes of beta-glucosidase in Dictyostelium discoideum.
    J Bacteriol. 1969 Dec;100(3):1208-17 PMID: 4982890
  17. Studies of lysosomal alpha-glucosidase. I. Purification and properties of the rat liver enzyme.
    Biochemistry. 1970 Mar 17;9(6):1403-15 PMID: 4313883
  18. Purification and properties of alpha-D-mannosidase from the limpet, Patella vulgata.
    Biochem J. 1970 Mar;117(1):129-37 PMID: 4986964
  19. Developmental regulation of alpha-mannosidase in Dictyostelium discoideum.
    J Bacteriol. 1970 Aug;103(2):375-81 PMID: 5464526
  20. Studies on N-acetyl-beta-D-glucosaminidase of Aspergillus oryzae. I. Purification and characterization of N-acetyl-beta-D-glucosaminidase obtained from Takadiastase.
    J Biochem. 1970 Jul;68(1):109-17 PMID: 5452757
  21. Studies on the glycosidases of jack bean meal. 3. Crystallization and properties of beta-N-acetylhexosaminidase.
    J Biol Chem. 1970 Oct 10;245(19):5153-60 PMID: 5506280
  22. The isolation and characterization of lysosomal particles from myxamoebae of the cellular slime mould Dictyostelium discoideum.
    Biochem J. 1970 Jul;118(3):505-12 PMID: 5528640
  23. [Enzymatic liberation of long-chain fatty acids from bacterial lipopolysaccharides with the aid of extracts from amoebae of Dictyostelium discoideum].
    Hoppe Seylers Z Physiol Chem. 1970 Sep;351(9):1123-32 PMID: 4920244
  24. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
    Biochem J. 1970 Sep;119(2):171-4 PMID: 5530748
  25. Bacteriolytic enzymes from Staphylococcus aureus. Specificity of ction of endo-beta-N-acetylglucosaminidase.
    Biochem J. 1970 Dec;120(4):735-44 PMID: 4250336
  26. [Enrichment and characterization of 2 forms of human N-acetyl-beta-D-hexosaminidase].
    Hoppe Seylers Z Physiol Chem. 1971 Aug;352(8):1119-33 PMID: 5098352
  27. Alpha-mannosidase from Phaseolus vulgaris. Purification and characterization.
    Eur J Biochem. 1972 Feb 15;25(2):308-14 PMID: 5039840
  28. Degradation of the murein-lipoprotein complex of Escherichia coli cell walls by Dictyostelium amoebae.
    Eur J Biochem. 1972 May;27(1):116-25 PMID: 4261814
  29. Enzyme synthesis in myxamoebae of the cellular slime mould Dictyostelium discoideum during growth in axenic culture.
    Biochem J. 1972 Feb;126(3):601-8 PMID: 4672668
  30. Enzyme synthesis in the cellular slime mould Dictyostelium discoideum during the differentiation of myxamoebae grown axenically.
    Biochem J. 1972 Feb;126(3):609-15 PMID: 4672669
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. The determination of enzyme inhibitor constants.
    Biochem J. 1953 Aug;55(1):170-1 PMID: 13093635
  33. Diffusion-in-gel methods for immunological analysis.
    Prog Allergy. 1958;5:1-78 PMID: 13578996
  34. Purification of beta-N-acetylglucosaminidase from the pig epididymis.
    Biochem J. 1960 Oct;77:170-5 PMID: 13699549
  35. Studies on glucosaminidase. 3. Testicular N-acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase.
    Biochem J. 1961 Jan;78:111-6 PMID: 13786642
  36. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  37. HISTOCHEMICAL DEMONSTRATION OF N-ACETYL-BETA-GLUCOSAMINIDASE EMPLOYING NAPHTHOL AS-BI N-ACETYL-BETA -GLUCOSAMINIDE AS SUBSTRATE.
    J Histochem Cytochem. 1965 May-Jun;13:355-60 PMID: 14344254
  38. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults.
    Biochem J. 1955 Dec;61(4):629-41 PMID: 13276348
  39. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
    J Biol Chem. 1965 Jun;240:2468-77 PMID: 14304855
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1330037
Published
1973-05-00
Pages
37-47
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177668
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1330037 PMC Full Text View journal details
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