Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.

The Biochemical journal ·Vol. 102 ·No. 2 ·1967-02-00 ·Pages 525-32

Robinson D, Price RG, Dance N

Abstract

1. The activities of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney have been compared when 4-methylumbelliferyl glycosides are used as substrates. 2. Separation by gel electrophoresis at pH7.0 indicated slow- and fast-moving components of rat-kidney beta-galactosidase. 3. The fast-moving component is also associated with the total beta-glucosidase activity and inhibition experiments indicate that a single enzyme species is responsible for both activities. 4. DEAE-cellulose chromatography and filtration on Sephadex gels suggests that the beta-glucosidase component is a small acidic molecule, of molecular weight approx. 40000-50000, with optimum pH5.5-6.0 for beta-galactosidase and beta-glucosidase activities. 5. The major beta-galactosidase component has low electrophoretic mobility, a calculated molecular weight of 80000 and optimum pH3.7.

MeSH Terms

Animals Chemical Phenomena Chemistry Chromatography Chromatography, Gel Electrophoresis Galactosidases/analysis Glucosidases/analysis Glucuronidase/analysis Glycoside Hydrolases/analysis Hydrogen-Ion Concentration In Vitro Techniques Kidney/enzymology Male Rats

Chemicals

Galactosidases Glucosidases Glycoside Hydrolases Glucuronidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Robinson D

Price R G

Dance N

References (25)

25 references, click to expand

Tissue fractionation studies. 15. Intracellular distribution and properties of beta-N-acetylglucosaminidase and beta-galactosidase in rat liver.
Biochem J. 1960 Mar;74:450-6 PMID: 14444755
THE METABOLISM OF GLUCOCEREBROSIDES. I. PURIFICATION AND PROPERTIES OF A GLUCOCEREBROSIDE-CLEAVING ENZYME FROM SPLEEN TISSUE.
J Biol Chem. 1965 Jan;240:39-43 PMID: 14253443
Inhibition of glycosidases by aldonolactones of corresponding configuration.
Biochem J. 1957 Feb;65(2):389-95 PMID: 13403921
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
Biochim Biophys Acta. 1960 Mar 11;38:470-83 PMID: 13826559
Histochemical demonstration of beta-D-galactosidase in the rat.
J Histochem Cytochem. 1958 Mar;6(2):122-9 PMID: 13514055
Mammalian glycosidases. 4. The intracellular localization of beta-galactosidase, alpha-mannosidase, beta-N-acetylglucosaminidase and alpha-L-fucosidase in mammalian tissues.
Biochem J. 1963 May;87:354-61 PMID: 14022534
The fluorimetric estimation of beta-glucuronidase in blood plasma.
Clin Chim Acta. 1965 Dec;12(6):659-70 PMID: 5880047
Studies on glucosaminidase; N-acetyl-beta-glucosaminidase in rat kidney.
Biochem J. 1957 Mar;65(3):464-9 PMID: 13412648
Potentiometric determination of chloride in biological fluids.
Biochem J. 1952 Nov;52(3):502-5 PMID: 13018266
RAT-KIDNEY LYSOSOMES: ISOLATION AND PROPERTIES.
Biochem J. 1965 Jun;95:731-41 PMID: 14342509
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
Biochem J. 1964 May;91(2):222-33 PMID: 4158310
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
Biochem J. 1965 Sep;96(3):595-606 PMID: 5862401
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
J Biol Chem. 1965 Jun;240:2468-77 PMID: 14304855
Specificity and multiple forms of beta-galactosidase in the rat.
Biochem J. 1965 Oct;97(1):59-66 PMID: 16749124
Pig intestinal beta-glucosidase activities. II. Evidence for the hydrolysis of 4-methylumbelliferyl beta-D-glucoside and beta-D-galactoside at the same enzyme site.
Biochim Biophys Acta. 1962 Oct 8;64:182-4 PMID: 13954348
Studies of small intestine during development. I. Distribution and activity of beta-galactosidase.
Biochim Biophys Acta. 1962 Aug 13;62:353-62 PMID: 13886893
Mammalian beta-galactosidases.
Biochem Biophys Res Commun. 1965 May 18;19(5):630-6 PMID: 4284242
FLUORIMETRIC DETERMINATION OF GLYCOSIDASES IN THE LOCUST (LOCUSTA MIGRATORIA) AND OTHER INSECTS.
Comp Biochem Physiol. 1964 May;12:95-105 PMID: 14195399
Action of neuraminidase on human kidney alkaline phosphatase.
Nature. 1966 Feb 19;209(5025):805-6 PMID: 5922152
Mammalian glycosidases; distribution in the body.
Biochem J. 1959 Feb;71(2):318-25 PMID: 13628572
Identification of the abnormal polypeptide chain of hemoglobin G-Ib.
J Mol Biol. 1960 Dec;2:372-8 PMID: 13703278
Studies on beta-glucuronidase. II. The preparation and properties of three-ox-spleen beta-glucuronidase fractions.
Biochem J. 1953 Jan;53(2):232-45 PMID: 13032060
A comparison of the beta-D-glucosidase and beta-D-galactosidase activities from eleven enzyme sources.
Comp Biochem Physiol. 1966 Jan;17(1):129-38 PMID: 5940078
The fluorimetric determination of beta-glucosidase: its occurrence in the tissues of animals, including insects.
Biochem J. 1956 May;63(1):39-44 PMID: 13315244
Studies on glucosaminidase. 4. The fluorimetric assay of N-acetyl-beta-glucosaminidase.
Biochem J. 1961 Jan;78:151-6 PMID: 13759894

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1020525

Published

1967-02-00

Pages

525-32

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270276

Subset

External Links

PubMed source DOI: 10.1042/bj1020525 PMC Full Text View journal details

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Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.

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