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PMID: 4195608 Published · ppublish English Journal Article

Plaque size heterogeneity: a genetic trait of lymphocytic choriomeningitis virus.

Applied microbiology ·Vol. 20 ·No. 1 ·1970-07-00 ·Pages 123-8

Pulkkinen AJ, Pfau CJ

Abstract

All of the ten strains of lymphocytic choriomeningitis virus assayed on BHK 21/13S cells showed various degrees of plaque size heterogeneity. The amount of virus released from these plaques was usually very small because of rapid photodynamic inactivation by neutral red. When virus from large and small plaques of a specific strain was plated, the same distribution of plaque size was obtained from each clone. Although it was shown that surface virus could possibly be randomly distributed at the time of addition of neutral red overlays, no virus could be isolated from nonplaque areas. Two different strains of virus (CA1371 and WE) with markedly different plaque size ranges were separated by plaque excision from plates infected with a mixture of both viruses.

MeSH Terms
Animals Cell Line Coloring Agents/pharmacology Cricetinae Culture Media Drug Resistance, Microbial Genetics, Microbial Kidney Light Lymphocytic choriomeningitis virus/drug effects,growth & development,pathogenicity Mice Polysaccharides Species Specificity Staining and Labeling Virus Cultivation Viruses, Unclassified/growth & development
Chemicals
Coloring Agents Culture Media Polysaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pulkkinen A J
Pfau C J
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22 references, click to expand
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Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.20.1.123-128.1970
Published
1970-07-00
Pages
123-8
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC376879
Subset
IM
External Links
PubMed source DOI: 10.1128/am.20.1.123-128.1970 PMC Full Text
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