Attachment of carbohydrate to the variable region of myeloma immunoglobulin light chains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 3 ·1970-07-00 ·Pages 975-82

Sox HC, Hood L

Abstract

Approximately 15 per cent of the light chains from homogeneous immunoglobulins in patients with multiple myeloma contain an oligosaccharide group. Five human myeloma light chains that contained carbohydrate were studied. The sequence Asn-[unk]-Ser/Thr was at the site of carbohydrate attachment in all light chains. The carbohydrate group was attached to the asparagine residue of this triplet sequence which in all five light chains was located in the variable region. The occasional occurrence of carbohydrate in myeloma light chains is seen as the consequence of a variable region mutation creating an Asn-[unk]-Ser/Thr sequence to which carbohydrate is attached by an enzyme capable of recognizing the characteristic triplet sequence.

MeSH Terms

Amino Acid Sequence Bence Jones Protein/urine Carbohydrates/analysis Glucosamine/analysis Glycoproteins/analysis Hexosamines/analysis Humans Immunoglobulins/analysis Multiple Myeloma/immunology,urine Neuraminic Acids/analysis Peptides/analysis,isolation & purification gamma-Globulins/analysis

Chemicals

Carbohydrates Glycoproteins Hexosamines Immunoglobulins Neuraminic Acids Peptides gamma-Globulins Bence Jones Protein Glucosamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sox H C

Hood L

References (19)

19 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.3.975

Published

1970-07-00

Pages

975-82

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283146

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.3.975 PMC Full Text View journal details

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Attachment of carbohydrate to the variable region of myeloma immunoglobulin light chains.

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