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PMID: 4141598 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro system for production of mouse mammary tumor virus.

Applied microbiology ·Vol. 28 ·No. 6 ·1974-12-00 ·Pages 1040-6

Fine DL, Arthur LO, PLOWMAN JK, Hillman EA, Klein F

Abstract

An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C(3)H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 x 10(10) particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.

MeSH Terms
Animals Cell Line Dexamethasone/pharmacology Fluorescent Antibody Technique Guanosine Triphosphate/metabolism Idoxuridine/pharmacology Mammary Neoplasms, Experimental Mammary Tumor Virus, Mouse/growth & development,immunology,isolation & purification Mice Mice, Inbred C3H Microscopy, Electron RNA-Directed DNA Polymerase/metabolism Thymine Nucleotides/metabolism Tritium Ultracentrifugation Uridine/metabolism Virus Cultivation Virus Replication/drug effects
Chemicals
Thymine Nucleotides Tritium Dexamethasone Guanosine Triphosphate RNA-Directed DNA Polymerase Idoxuridine Uridine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fine D L
Arthur L O
PLOWMAN J K
Hillman E A
Klein F
References (22)
22 references, click to expand
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Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.28.6.1040-1046.1974
Published
1974-12-00
Pages
1040-6
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC186879
Subset
IM
External Links
PubMed source DOI: 10.1128/am.28.6.1040-1046.1974 PMC Full Text
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