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PMID: 4098102 Published · ppublish English Comparative Study Journal Article

Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.

Applied microbiology ·Vol. 20 ·No. 2 ·1970-08-00 ·Pages 259-62

Monroe JH, Brandt PM

Abstract

A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.

MeSH Terms
Herpesviridae/isolation & purification Herpesvirus 4, Human/isolation & purification Latex Leukemia Virus, Murine/isolation & purification Methods Microscopy, Electron Microspheres Phosphotungstic Acid Rauscher Virus/isolation & purification Serum Albumin, Bovine Simplexvirus/isolation & purification Staining and Labeling
Chemicals
Latex Phosphotungstic Acid Serum Albumin, Bovine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Monroe J H
Brandt P M
References (10)
10 references, click to expand
  1. Herpes simplex virus: dry mass.
    Science. 1969 Nov 28;166(3909):1163-5 PMID: 4310570
  2. Electron densitometry of stained virus particles.
    J Biophys Biochem Cytol. 1955 Jan;1(1):1-12 PMID: 14381423
  3. Complete and coreless forms of reovirus (ECHO 10). Ratio of number of virus particles to infective units in the one-step growth cycle.
    Virology. 1961 Dec;15:428-35 PMID: 14491771
  4. Electron microscopic counting of virus particles by sedimentation on aluminized grids.
    J Immunol. 1962 Aug;89:279-84 PMID: 13914320
  5. Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique.
    Virology. 1963 Mar;19:250-60 PMID: 13999204
  6. Electron-microgrophic particle counts of phosphotungstate-sprayed virus.
    Biochim Biophys Acta. 1962 Sep 17;61:321-31 PMID: 13999205
  7. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).
    Lancet. 1964 Feb 1;1(7327):238-40 PMID: 14086209
  8. APPLICATION OF IMMUNOFLUORESCENCE TO THE STUDY OF HUMAN LEUKEMIA.
    J Natl Cancer Inst. 1964 Sep;33:581-8 PMID: 14207865
  9. Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations.
    J Natl Cancer Inst. 1960 Apr;24:933-51 PMID: 14423465
  10. A negative staining method for high resolution electron microscopy of viruses.
    Biochim Biophys Acta. 1959 Jul;34:103-10 PMID: 13804200
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.20.2.259-262.1970
Published
1970-08-00
Pages
259-62
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC376912
Subset
IM
External Links
PubMed source DOI: 10.1128/am.20.2.259-262.1970 PMC Full Text
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