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PMID: 4137106 Published · ppublish English Journal Article

Physiological and morphological identification of hypothalamic magnocellular neuroendocrine cells in goldfish preoptic nucleus.

The Journal of physiology ·Vol. 239 ·No. 1 ·1974-05-00 ·Pages 103-24

Hayward JN

Abstract

1. Intracellular recordings were made from antidromically identified neurones in the goldfish preoptic nucleus and Procion Yellow was ejected from the recording pipette, marking these magnocellular neuroendocrine cells diffusely, for histological identification.2. In confirmation of earlier studies these preoptic neuroendocrine cells showed resting membrane potentials of 47 mV, action potentials up to 85 mV, action potentials of long duration (3.9 msec) occurring in two steps, long-lasting hyperpolarizing after-potentials and orthodromic driving from olfactory input.3. Magnocellular neuroendocrine cells exhibited all-or-none jumps to shorter antidromic latencies as pituitary stimulus strength increased, suggesting multiple branching of the ;axones' either near the preoptic nucleus or within the neural lobe.4. I find three morphological types of neuroendocrine cells throughout the magnocellular part of the preoptic nucleus. Cell Type I is a large (37 mum), multipolar neurone, 48 mum from the ependyma, with fine ;dendrites' projecting into the lateral hypothalamus and within the preoptic nucleus, with multiple branched ;axones'. Cell Type II is a large (31 mum), multipolar neurone, 24 mum from the ependyma, with a coarse ;dendrite' to the ependyma and fine ;dendrites' within the preoptic nucleus, with limited ;axonal' branching. Cell Type III is a small (18 mum), multipolar neurone, 46 mum from the ependyma, with fine ;dendritic' processes distributed within the preoptic nucleus, with limited ;axonal' branching.5. I conclude that magnocellular neuroendocrine cells show electrical membrane properties of other central neurones with both physiological and morphological evidence for multiple ;axonal' branching. The three identifiable neuroendocrine cell types (I, II, III) are distributed widely within the anatomical limits of the preoptic nucleus, pars magnocellularis, with each type receiving ;specific' input connexions and with unique output pathways. I suggest that these three types of neuroendocrine cells may be related to the ;cellular' secretion of ;specific' neurohypophysial hormones and neurophysins.

MeSH Terms
Action Potentials Animals Axons Biometry Brain Mapping Coloring Agents Cyprinidae/anatomy & histology Dendrites Electric Stimulation Hypothalamus/cytology,physiology Limbic System/physiology Membrane Potentials Neurosecretory Systems/cytology Olfactory Pathways Pituitary Gland/physiology Staining and Labeling
Chemicals
Coloring Agents
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hayward J N
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1974.sp010558
Published
1974-05-00
Pages
103-24
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1330940
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1974.sp010558 PMC Full Text View journal details
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