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PMID: 4133851 Published · ppublish English Comparative Study Journal Article

Structural and functional similarities between mitochondrial malate dehydrogenase and L-3-hydroxyacyl CoA dehydrogenase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1334-8

Noyes BE, Glatthaar BE, Garavelli JS, Bradshaw RA

Abstract

Pig heart mitochondrial malate dehydrogenase (EC 1.1.1.37), which has been obtained free of electrophoretic subforms, has been shown to have a molecular weight of 67,000 and to be composed of two polypeptide chains. Comparison of these and other properties, such as amino-acid composition, isoelectric point, and keto-substrate inhibition, with those of (L)-3-hydroxyaeyl CoA dehydrogenase (EC 1.1.1.35), another NAD(+)-dependent dehydrogenase of mitochondrial origin, suggests structural similarities of the type associated with proteins possessing common evolutionary origins. This conclusion is supported by immunological crossreactivity. In view of these observations, the dissimilarity in the stereospecificity of hydrogen transfer from cofactor to substrate catalyzed by the two enzymes is attributed to 180 degrees rotation in the binding orientation of the nicotinamide moiety of the NAD(+), rather than to gross differences in the geometry of the active site of the two enzymes.

MeSH Terms
Alcohol Oxidoreductases/analysis,metabolism Amino Acid Sequence Amino Acids/analysis Animals Coenzyme A Cross Reactions Cytoplasm/enzymology Epitopes Isoelectric Focusing Malate Dehydrogenase/analysis,metabolism Mitochondria, Muscle/enzymology Molecular Weight Myocardium/enzymology Precipitin Tests Protein Conformation Structure-Activity Relationship Swine
Chemicals
Amino Acids Epitopes Alcohol Oxidoreductases Malate Dehydrogenase Coenzyme A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Noyes B E
Glatthaar B E
Garavelli J S
Bradshaw R A
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1334
Published
1974-04-00
Pages
1334-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388222
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1334 PMC Full Text View journal details
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