A reappraisal of some structural features of bovine heart malate dehydrogenase.

The Biochemical journal ·Vol. 109 ·No. 4 ·1968-10-00 ·Pages 663-8

Heyde E, Ainsworth S

Abstract

1. Malate dehydrogenase of the mitochondrial type was prepared from an acetone-prepared powder of thoroughly washed minces of whole bovine heart by previously reported methods that were modified to give higher yields of the purified enzyme. 2. Determinations of the sedimentation and diffusion coefficients showed the molecular weight of the enzyme to be approx. 63000. The amino acid composition of the enzyme was also determined. Discrepancies between these data and similar data previously reported by Davies & Kun (1957) and Siegel & Englard (1962) were resolved. 3. ;Fingerprints' were made from tryptic digests of heat-denatured and of reduced and alkylated enzyme. These indicated that the enzyme is composed of a number of identical or similar sub-units.

MeSH Terms

Amino Acids/analysis Animals Cattle Digestion Malate Dehydrogenase/analysis,isolation & purification Molecular Weight Myocardium/analysis Trypsin Ultracentrifugation

Chemicals

Amino Acids Malate Dehydrogenase Trypsin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Heyde E

Ainsworth S

References (14)

14 references, click to expand

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The active centre of triose phosphate isomerase.
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Some molecular and kinetic properties of heart malic dehydrogenase.
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J Biol Chem. 1961 Jul;236:1980-5 PMID: 13708709
Beef-heartmalic dehydrogenases. II. Preparation and properties of crystalline supernatant malic dehydrogenase.
Biochim Biophys Acta. 1962 Jan 29;56:571-83 PMID: 13890275
Beef-heart malic dehydrogenases. I. Properties of the enzyme purified from extracts of acetone-dried powders.
Biochim Biophys Acta. 1961 Nov 25;54:67-76 PMID: 13912628
Beef-heart malic dehydrogenases. III. Comparative studies of some properties of M-malic dehydrogenase and S-malic dehydrogenase.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1090663

Published

1968-10-00

Pages

663-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186952

Subset

External Links

PubMed source DOI: 10.1042/bj1090663 PMC Full Text View journal details

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A reappraisal of some structural features of bovine heart malate dehydrogenase.

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