Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 11 ·1973-11-00 ·Pages 3125-9

Levy NL, Synderman R, Ladda RL, Lieberman R

Abstract

Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5-Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic "repair" in mammals.

MeSH Terms

Animals Cell Fusion Cells, Cultured Chemotaxis Complement System Proteins/analysis Epitopes Erythrocytes Fibroblasts Genetic Engineering Guinea Pigs Hemolysis Histamine Release Hybrid Cells/transplantation Immunoassay Immunodiffusion Immunologic Deficiency Syndromes/genetics Kidney Macrophages Parainfluenza Virus 1, Human Species Specificity Spleen Transplantation, Heterologous Transplantation, Homologous

Chemicals

Epitopes Complement System Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Levy N L

Synderman R

Ladda R L

Lieberman R

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.11.3125

Published

1973-11-00

Pages

3125-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427184

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.11.3125 PMC Full Text View journal details

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Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.

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