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PMID: 4332008 Published · ppublish English Journal Article

Genetics of somatic mammalian cells: lethal antigens as genetic markers for study of human linkage groups.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3102-6

Puck TT, Wuthier P, Jones C, Kao FT

Abstract

The antigen that causes killing of at least 98% of a human cell population treated with a 1% solution of a specific rabbit antiserum in the presence of complement is a sensitive genetic marker. The rapid loss of human chromosomes in human-Chinese hamster cell hybrids makes possible a convenient test of linkage relationships with this marker. Hybrid clones with and without the lethal antigen were isolated and analyzed. In 76 clones and subclones studied, 41 carried both the lethal antigen and the lactic dehydrogenase-A marker, 35 carried neither, and no clones contained only one of the two markers. In contrast to this clear demonstration of linkage, absence of linkage was found between the lethal antigen and the following markers: Lactic dehydrogenase B, NAD-dependent malic dehydrogenase, NADP-dependent malic dehydrogenase, glucose-6-phosphate dehydrogenase, phosphoglucomutase, glutamate oxaloacetate transaminase, indophenol oxidase, glucose phosphate isomerase, proline, inositol, hypoxanthine B, and glycine A. This lethal antigen appears to be carried on a single human autosome.

MeSH Terms
Animals Antigens Binding Sites Cell Fusion Chromosome Mapping Clone Cells Complement System Proteins Cricetinae Cytogenetics Genes, Lethal Genetic Linkage Humans Hybrid Cells Hybridization, Genetic Immune Sera Isoenzymes L-Lactate Dehydrogenase Mutation Parainfluenza Virus 1, Human/radiation effects Radiation Effects Species Specificity Ultraviolet Rays
Chemicals
Antigens Immune Sera Isoenzymes Complement System Proteins L-Lactate Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Puck T T
Wuthier P
Jones C
Kao F T
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.12.3102
Published
1971-12-00
Pages
3102-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389599
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.12.3102 PMC Full Text View journal details
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