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PMID: 4123687 Published · ppublish English Journal Article

A new complement-mediated cytolytic mechanism--the C1-bypass activation pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 3 ·1973-03-00 ·Pages 649-52

May JE, Frank MM

Abstract

A new cytolytic pathway is described whereby cells sensitized with cytotoxic antibody can be specifically lysed in the complete absence of intact, classical complement pathway function. Evaluation of this model with sera deficient in the 4th (C4), 2nd (C2), and 6th (C6) components of complement has revealed that this new lytic mechanism, termed the C1-bypass activation pathway, is initiated by the antibody-mediated activation of C1. Utilizing components of the previously described alternate complement pathway, this pathway bypasses C4 and C2 to activate the third to ninth components of complement (C3-9) with induction of membrane damage.

MeSH Terms
Animals Antibodies Complement System Proteins Cytotoxicity Tests, Immunologic Forssman Antigen Guinea Pigs Hemolysis Humans Immunoelectrophoresis Immunologic Deficiency Syndromes/immunology Sheep/immunology gamma-Globulins
Chemicals
Antibodies gamma-Globulins Complement System Proteins Forssman Antigen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
May J E
Frank M M
References (21)
21 references, click to expand
  1. ESTIMATION OF MOLECULAR SIZE OF COMPLEMENT COMPONENTS BY SEPHADEX CHROMATOGRAPHY.
    J Immunol. 1965 Apr;94:510-3 PMID: 14299023
  2. The properdin system and immunity. IV. The hemolysis of erythrocytes from patients with paroxysmal nocturnal hemoglobinuria.
    J Clin Invest. 1956 May;35(5):453-7 PMID: 13319478
  3. Deficiency of the sixth component of complement in rabbits with an inherited complement defect.
    J Exp Med. 1966 Oct 1;124(4):773-85 PMID: 5922290
  4. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.
    Immunochemistry. 1966 Mar;3(2):111-35 PMID: 5960883
  5. Complement components of a haemolytically deficient strain of rabbits.
    Immunology. 1968 Apr;14(4):527-40 PMID: 5653813
  6. Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components.
    J Exp Med. 1968 Nov 1;128(5):1049-57 PMID: 4176223
  7. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.
    Immunochemistry. 1969 Jan;6(1):53-66 PMID: 4179831
  8. The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.
    J Exp Med. 1969 Nov 1;130(5):1145-60 PMID: 5347695
  9. The biologic activities of guinea pig antibodies. II. Modes of complement interaction with gamma 1 and gamma 2-immunoglobulins.
    J Immunol. 1970 Feb;104(2):329-34 PMID: 4189670
  10. Genetically controlled total deficiency of the fourth component of complement in the guinea pig.
    Science. 1970 Oct 2;170(3953):74-5 PMID: 4989151
  11. In vitro studies of complement function in sera of C4-deficient guinea pigs.
    J Exp Med. 1971 Jul 1;134(1):176-87 PMID: 4997584
  12. The C3-activator system: an alternate pathway of complement activation.
    J Exp Med. 1971 Sep 1;134(3 Pt 2):90s-108s PMID: 4105810
  13. An alternate complement pathway: C-3 cleaving activity, not due to C4,2a, on endotoxic lipopolysaccharide after treatment with guinea pig serum; relation to properdin.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1351-4 PMID: 5288385
  14. Paroxysmal nocturnal hemoglobinuria. Hemolysis initiated by the C3 activator system.
    N Engl J Med. 1972 Jan 27;286(4):180-4 PMID: 5007123
  15. C1r deficiency: an inborn error associated with cutaneous and renal disease.
    J Clin Invest. 1972 May;51(5):1102-8 PMID: 4623164
  16. Production of chemotactic activity by guinea pig immunoglobulins following activation of the C3 complement shunt pathway.
    J Immunol. 1972 May;108(5):1227-31 PMID: 5029138
  17. Complement-mediated tissue damage: contribution of the classical and alternate complement pathways in the Forssman reaction.
    J Immunol. 1972 Jun;108(6):1517-25 PMID: 4537434
  18. Heat labile opsonins to Pneumococcus. 3. The participation of immunoglobulin and of the alternate pathway of C3 activation.
    J Immunol. 1972 Jun;108(6):1681-9 PMID: 4402312
  19. The alternate complement pathway in cell damage: antibody-mediated cytolysis of erythrocytes and nucleated cells.
    J Immunol. 1972 Sep;109(3):595-601 PMID: 4115545
  20. C3 shunt activation in human serum chelated with EGTA.
    J Immunol. 1972 Oct;109(4):807-9 PMID: 4627510
  21. Hereditary deficiency of the second component of complement (C'2) in man.
    J Clin Invest. 1966 Jun;45(6):880-90 PMID: 4161820
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.3.649
Published
1973-03-00
Pages
649-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433326
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.3.649 PMC Full Text View journal details
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