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PMID: 4117351 Published · ppublish English Journal Article

Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus.

Journal of virology ·Vol. 10 ·No. 4 ·1972-10-00 ·Pages 824-34

Kelly RK, Loh PC

Abstract

An electron microscopical and biochemical examination of the properties of infectious pancreatic necrosis virus (IPN) and of its ribonucleic acid (RNA) was made. The buoyant density of IPN in CsCl was found to be 1.33 g/cm(3). Electron microscopical examination of the banded virus revealed structures similar in size (74 nm) and shape to reoviruses but lacking a characteristic inner capsid structure. Polyacrylamide gel electrophoretic analysis of IPN-RNA revealed a single non-segmented component of molecular weight 3.2 x 10(6). Its susceptibility to ribonuclease, base composition, and resistance to thermal denaturation indicated a single-stranded RNA structure. However, its sedimentation behavior (16S) independent of ionic strength in sucrose gradients, partial solubility in 2 m LiCl, and ribonuclease resistance in the presence of Mg(2+) suggest an unusual secondary structure of unknown nature. The accumulated data indicate that IPN virus does not belong to either the picornavirus or reovirus groups and may represent a new group of viruses.

MeSH Terms
Animals Cell Line Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Electrophoresis, Polyacrylamide Gel Fish Diseases/microbiology Gonads Hot Temperature Microscopy, Electron Molecular Weight Nucleic Acid Denaturation Nucleotides/analysis Phosphorus Isotopes Picornaviridae/analysis,isolation & purification RNA Viruses/classification RNA, Viral/analysis,isolation & purification Ribonucleases Salmonidae Staining and Labeling Tritium Viral Proteins
Chemicals
Nucleotides Phosphorus Isotopes RNA, Viral Viral Proteins Tritium Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelly R K
Loh P C
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.4.824-834.1972
Published
1972-10-00
Pages
824-34
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356539
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.4.824-834.1972 PMC Full Text View journal details
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