A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. II. Reduction of TSP-1-specific receptor sites on cells grown at 37 C or 45 C, and the temperature-dependent inactivation of replicating phage.

Journal of virology ·Vol. 9 ·No. 4 ·1972-04-00 ·Pages 652-8

LaMontagne JR, McDonald WC

Abstract

The adsorption of subtilisphage TSP-1 to Bacillus subtilis W168 was tested with cells grown at 37, 45, and 53 C. Kinetic data, electron micrographs, and quantitative measurement of the per cent phage adsorbed all indicated that irreversible adsorption occurs only with cells grown at 53 C. If TSP-1 was allowed to infect cells at 53 C, subsequent shifts to 37 C inhibited phage replication and resulted in the inactivation of plaque-forming units.

MeSH Terms

Adsorption Animals Bacillus subtilis Bacteriophages/growth & development,immunology Carbon Isotopes DNA, Viral Hot Temperature Immune Sera Isoleucine Kinetics Microscopy, Electron Neutralization Tests Phosphates Phosphorus Isotopes Rabbits/immunology Soil Microbiology Staining and Labeling Temperature Virus Replication

Chemicals

Carbon Isotopes DNA, Viral Immune Sera Phosphates Phosphorus Isotopes Isoleucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

LaMontagne J R

McDonald W C

References (8)

8 references, click to expand

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J Bacteriol. 1969 Jun;98(3):1087-97 PMID: 4977981
THE STRUCTURE OF BACTERIOPHAGE SP8.
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Morphological changes and antibiotic-induced thermal resistance in vegetative cells of Bacillus subtilis.
J Bacteriol. 1971 May;106(2):672-8 PMID: 4995654
A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. I. Physical and chemical characteristics of TSP-1.
J Virol. 1972 Apr;9(4):646-51 PMID: 4112068
CAPSOMERES AND OTHER STRUCTURES OBSERVED ON SOME BACTERIOPHAGES.
Pathol Microbiol (Basel). 1965;28:175-80 PMID: 14299862
Alteration of host specificity in Bacillus subtilis.
Bacteriol Rev. 1968 Dec;32(4 Pt 1):297-301 PMID: 4974084
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
Independent functions of viral protein and nucleic acid in growth of bacteriophage.
J Gen Physiol. 1952 May;36(1):39-56 PMID: 12981234

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.4.652-658.1972

Published

1972-04-00

Pages

652-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356350

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.4.652-658.1972 PMC Full Text View journal details

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A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. II. Reduction of TSP-1-specific receptor sites on cells grown at 37 C or 45 C, and the temperature-dependent inactivation of replicating phage.

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