A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. I. Physical and chemical characteristics of TSP-1.

Journal of virology ·Vol. 9 ·No. 4 ·1972-04-00 ·Pages 646-51

LaMontagne JR, McDonald WC

Abstract

Bacteriophage TSP-1 was isolated from soil in a search for phage which would form plaques on Bacillus subtilis W168 at 53 C. It forms clear plaques only at temperatures from 50 to 55 C. Approximately 95% of the free phage adsorb after 2 min at 53 C. The lytic cycle is between 55 and 60 min long with a burst size of about 55 particles per infected bacterium. The phage was shown to contain double-stranded deoxyribonucleic acid with a base composition of 44.7% guanine plus cytosine. This deoxyribonucleic acid does not contain a base analogue for thymine and has a molecular weight estimated at 56 x 10(6) daltons.

MeSH Terms

Adsorption Bacillus subtilis Bacteriolysis Bacteriophages/analysis,growth & development,isolation & purification Centrifugation, Density Gradient Cesium Chlorides Chromatography, Paper Cytosine/analysis DNA, Viral/analysis Guanine/analysis Hot Temperature Microscopy, Electron Molecular Weight Phosphorus Isotopes Soil Microbiology Spectrophotometry Staining and Labeling Time Factors Viral Proteins Virus Cultivation Virus Replication

Chemicals

Chlorides DNA, Viral Phosphorus Isotopes Viral Proteins Cesium Guanine Cytosine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

LaMontagne J R

McDonald W C

References (11)

11 references, click to expand

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Morphological changes and antibiotic-induced thermal resistance in vegetative cells of Bacillus subtilis.
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CAPSOMERES AND OTHER STRUCTURES OBSERVED ON SOME BACTERIOPHAGES.
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.4.646-651.1972

Published

1972-04-00

Pages

646-51

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356349

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.4.646-651.1972 PMC Full Text View journal details

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A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. I. Physical and chemical characteristics of TSP-1.

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