Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis.
The Mr 28.000 crystal protein gene of Bacillus thuringiensis subspecies israelensis has been cloned into pBR322 as part of a 9.7 kb HindIII fragment. From hybridization experiments of recombinant p425 DNA with B.t. subspecies israelensis RNA from different stages of growth it was concluded that transcription of the gene is restricted to early sporulation stages. Nucleotide sequence analysis revealed the presence of a large open reading frame with a coding capacity of 249 amino acids (Mr 27.340). Nuclease S1 mapping demonstrated that transcription starts 44 nucleotides upstream of the initiation codon. A Shine-Dalgarno sequence (AAGGAG) was found 10 nucleotides upstream of the translation startpoint. At the 3'-end of the gene a complex secondary structure was found immediately after the stop-codon. Despite the presence of these regulation signals only limited expression in E. coli was detected. This can be explained by assuming that B.t. subsp. israelensis promotor sequences are poorly recognized by E. coli RNA polymerase.
23 references, click to expand
-
Genes for Bacillus megaterium small, acid-soluble spore proteins: nucleotide sequence of two genes and their expression during sporulation.Gene. 1985;35(1-2):151-7 PMID: 3928443
-
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.Nucleic Acids Res. 1985 Apr 11;13(7):2251-65 PMID: 2987847
-
Presence of polyriboadenylate sequences in pulse-labeled RNA of Escherichia coli.Proc Natl Acad Sci U S A. 1975 Aug;72(8):2910-4 PMID: 1103127
-
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.Cell. 1977 Nov;12(3):721-32 PMID: 922889
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
Regulatory sequences involved in the promotion and termination of RNA transcription.Annu Rev Genet. 1979;13:319-53 PMID: 94251
-
Comparative biochemistry of entomocidal parasporal crystals of selected Bacillus thuringiensis strains.J Bacteriol. 1981 Feb;145(2):1052-62 PMID: 7462158
-
Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli.Proc Natl Acad Sci U S A. 1981 May;78(5):2893-7 PMID: 7019914
-
Prediction of protein antigenic determinants from amino acid sequences.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8 PMID: 6167991
-
Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 PMID: 6269055
-
Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.J Biol Chem. 1981 Nov 10;256(21):11283-91 PMID: 6793593
-
Molecular relationships among plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains.Mol Gen Genet. 1982;186(3):391-8 PMID: 6956789
-
Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene.J Biol Chem. 1983 Jan 25;258(2):1007-13 PMID: 6185474
-
Mechanism of action of Bacillus thuringiensis var israelensis insecticidal delta-endotoxin.FEBS Lett. 1983 Apr 18;154(2):362-8 PMID: 6832375
-
Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.Nature. 1983 Apr 28;302(5911):800-4 PMID: 6405278
-
A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis.Plasmid. 1984 Jan;11(1):28-38 PMID: 6709732
-
Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715.EMBO J. 1982;1(7):791-9 PMID: 6329704
-
Cloning and expression in Escherichia coli of the insecticidal delta-endotoxin gene of Bacillus thuringiensis var. israelensis.FEBS Lett. 1984 Oct 1;175(2):377-82 PMID: 6090216
-
Delta endotoxin of Bacillus thuringiensis subsp. israelensis.J Bacteriol. 1985 Jan;161(1):39-46 PMID: 2981808
-
Separation of the cytolytic and mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis.Biochem Biophys Res Commun. 1985 Jan 31;126(2):961-5 PMID: 2858208
-
Molecular cloning of the delta-endotoxin gene of Bacillus thuringiensis var. israelensis.Gene. 1985;33(2):151-8 PMID: 2987085
-
The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence.J Biol Chem. 1985 May 25;260(10):6264-72 PMID: 2581950
-
Improved estimation of secondary structure in ribonucleic acids.Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
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