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PMID: 4066929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular heterogeneity of histochemical fibre types: a comparison of fast fibres.

Journal of muscle research and cell motility ·Vol. 6 ·No. 4 ·1985-08-00 ·Pages 513-24

Moore GE, Schachat FH

Abstract

The relationship between histochemical fibre type and contractile protein expression was analysed in three rabbit skeletal muscles, the erector spinae, the plantaris and the diaphragm. A procedure for staining fibre bundles was developed using the same histochemical methods as those for typing fibres in cross-section. This allowed pretyped fibres to be selected and their molecular composition to be analysed by gel electrophoresis. The balance of expression of the two predominant fast troponin species, TnT1f and TnT2f, and alpha and beta tropomyosin subunits were studied in type IIA and IIB fast fibres. Type IIA fibres exhibited a restricted pattern of thin filament expression, exhibited TnT1f and both tropomyosin subunits in all three muscles. The expression in type IIB fibres, however, ranges from predominantly TnT2f and the alpha tropomyosin subunit in the erector spinae to TnT1f with both alpha and beta subunits in the diaphragm. These results indicate that there is not a simple one-to-one relationship between the fast muscle fibre subtypes and the expression of different thin filament protein isoforms.

MeSH Terms
Animals Diaphragm Electrophoresis, Polyacrylamide Gel Female Macromolecular Substances Muscle, Smooth/cytology Muscles/cytology Organ Specificity Rabbits Tropomyosin/analysis Troponin/analysis
Chemicals
Macromolecular Substances Tropomyosin Troponin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moore G E
Schachat F H
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34 references, click to expand
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Article Info
Journal
Journal of muscle research and cell motility
Abbr.
J Muscle Res Cell Motil
ISSN
0142-4319
DOI
10.1007/BF00712587
Published
1985-08-00
Pages
513-24
Language
English
Region
Netherlands
NLM ID
8006298
Subset
IM
Grants
NINDS NIH HHS · NS 18228 · United States
External Links
PubMed source DOI: 10.1007/BF00712587 View journal details
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