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PMID: 4044645 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Biochemical studies on cell fusion. I. Lipid composition of fusion-resistant cells.

The Journal of cell biology ·Vol. 101 ·No. 4 ·1985-10-00 ·Pages 1578-90

Roos DS, Choppin PW

Abstract

A series of stable cell mutants of mouse fibroblasts were previously isolated (Roos, D. S. and R. L. Davidson, 1980, Somatic Cell Genet., 6:381-390) that exhibit varying degrees of resistance to the fusion-inducing effect of polyethylene glycol (PEG), but are morphologically similar to the parental cells from which they were derived. Biochemical analysis of these mutant cell lines has revealed differences in whole cell lipid composition which are directly correlated with their susceptibility to fusion. Fusion-resistant cells contain elevated levels of neutral lipids, particularly triglycerides and an unusual ether-linked lipid, O-alkyl, diacylglycerol. This ether lipid is increased approximately 35-fold over parental cells in the most highly PEG-resistant cell line. Fusion-resistant cells also contain more highly saturated fatty acyl chains (ratio of saturated to polyunsaturated fatty acids [S/P ratio] approximately 4:1) than the parental line (S/P ratio approximately 1:1). Cells which are intermediate in their resistance to PEG have ether lipid and fatty acid composition which is intermediate between the parental cells and the most fusion-resistant mutants. In a related communication (Roos, D. S. and P. W. Choppin, 1985, J. Cell. Biol., 100:1591-1598) evidence is presented that alteration of lipid content can predictably control the fusion response of these cells.

MeSH Terms
Animals Cell Fusion/drug effects Chromatography, Thin Layer Diglycerides/analysis Fatty Acids/analysis L Cells/analysis,drug effects,physiology Lipids/analysis,classification Mice Phospholipids/analysis Plasmalogens/analysis Polyethylene Glycols/pharmacology
Chemicals
Diglycerides Fatty Acids Lipids Phospholipids Plasmalogens Polyethylene Glycols
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roos D S
Choppin P W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.4.1578
Published
1985-10-00
Pages
1578-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113923
Subset
IM
Grants
NIAID NIH HHS · AI-05600 · United States
NIAID NIH HHS · AI-07233 · United States
NCI NIH HHS · CA-18213 · United States
External Links
PubMed source DOI: 10.1083/jcb.101.4.1578 PMC Full Text View journal details
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