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PMID: 4020971 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

mRNA activity of a Sindbis virus defective-interfering RNA.

Journal of virology ·Vol. 55 ·No. 3 ·1985-09-00 ·Pages 877-80

Migliaccio G, Castagnola P, Leone A, Cerasuolo A, Bonatti S

Abstract

We obtained Sindbis defective-interfering particles by nine and undiluted passages of standard virus on chicken embryo fibroblasts. These particles contain a deleted 20S RNA molecule which has mRNA activity, as shown by translation in cell-free systems in vitro. In infected cells, this mRNA activity appeared to be totally inhibited except at very late times postinfection.

MeSH Terms

Defective Viruses/genetics Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Sindbis Virus/genetics

Chemicals

RNA, Messenger RNA, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Migliaccio G

Castagnola P

Leone A

Cerasuolo A

Bonatti S

References (13)

13 references, click to expand

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Defective-interfering particles of Semliki Forest virus: intracellular events during interference.
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RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
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Membrane biogenesis. In vitro cleavage, core glycosylation, and integration into microsomal membranes of sindbis virus glycoproteins.
J Cell Biol. 1979 Jan;80(1):219-24 PMID: 422651
Oligonucleotide mapping studies of standard and defective Sindbis virus RNA.
J Virol. 1979 Feb;29(2):794-8 PMID: 430611
Restricted initiation of protein synthesis on the potentially polycistronic Sindbis virus 42 S RNA.
J Biol Chem. 1980 Dec 10;255(23):11473-7 PMID: 7440550
Complete nucleotide sequence of the genomic RNA of Sindbis virus.
Virology. 1984 Feb;133(1):92-110 PMID: 6322438
Sequence analysis of cDNA's derived from the RNA of Sindbis virions and of defective interfering particles.
J Virol. 1982 Jan;41(1):153-62 PMID: 6896345
RNAs from two independently isolated defective interfering particles of Sindbis virus contain a cellular tRNA sequence at their 5' ends.
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3279-83 PMID: 6304704
Biosynthesis of Sindbis virus membrane glycoproteins in vitro.
Methods Enzymol. 1983;96:512-9 PMID: 6361461
Infection of neuroblastoma cells by Semliki Forest virus. The interference of viral capsid protein with the binding of host messenger RNAs into initiation complexes is the cause of the shut-off of host protein synthesis.
Eur J Biochem. 1984 Feb 1;138(3):473-8 PMID: 6692830
Common and distinct regions of defective-interfering RNAs of Sindbis virus.
J Virol. 1984 Mar;49(3):865-72 PMID: 6699940
Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062-6 PMID: 6941270

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.55.3.877-880.1985

Published

1985-09-00

Pages

877-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC255080

Subset

External Links

PubMed source DOI: 10.1128/JVI.55.3.877-880.1985 PMC Full Text View journal details

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