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PMID: 4008046 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of human neutrophil chemiluminescence by plasmid-mediated outer membrane proteins of Yersinia enterocolitica.

Infection and immunity ·Vol. 49 ·No. 1 ·1985-07-00 ·Pages 145-51

Lian CJ, Pai CH

Abstract

Recent studies have shown that the cell surface properties of Yersinia enterocolitica are altered by the presence of the virulence plasmid, which mediates temperature-inducible outer membrane proteins (OMP). We investigated the interaction of Y. enterocolitica with human polymorphonuclear leukocytes by monitoring luminol-enhanced chemiluminescence (CL) responses. A plasmid-bearing strain grown at 37 degrees C induced four- to sixfold less CL than did the same strain grown at 25 degrees C or a plasmidless, isogenic strain grown at either temperature. Inhibition of CL responses by whole cells was related to plasmid-mediated expression of OMP. The OMP alone could inhibit the CL response of polymorphonuclear leukocytes stimulated by either opsonized zymosan or whole cells of Y. enterocolitica. Pronase treatment of whole cells, which removed the plasmid-mediated OMP, resulted in partial but significant elimination of CL inhibition by whole cells and by OMP derived from them. Incubation with Y. enterocolitica for 60 min did not affect the viability of polymorphonuclear leukocytes. Our results suggest that the interaction of Y. enterocolitica with human polymorphonuclear leukocytes is directly affected by the plasmid-mediated OMP.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,immunology Cell Survival Humans Luminescent Measurements Luminol/metabolism Neutrophils/immunology Plasmids Pronase Temperature Yersinia enterocolitica/genetics,pathogenicity
Chemicals
Bacterial Outer Membrane Proteins Luminol Pronase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lian C J
Pai C H
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45 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.1.145-151.1985
Published
1985-07-00
Pages
145-51
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262071
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.1.145-151.1985 PMC Full Text View journal details
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