Home LiteratureArticle Details

PMID: 3968041 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 786-8

Hinton JC, Perombelon MC, Salmond GP

Abstract

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid analysis. ColE1-based plasmids make potentially useful cloning vectors for the study of genes involved in the pathogenesis of this species.

MeSH Terms

Erwinia/genetics Plasmids Transformation, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hinton J C

Perombelon M C

Salmond G P

References (16)

16 references, click to expand

Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
Frozen-thawed bacteria as recipients of isolated coliphage DNA.
Biochim Biophys Acta. 1972 Oct 27;281(3):319-23 PMID: 4565238
Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination.
J Mol Biol. 1975 Aug 15;96(3):495-509 PMID: 1100850
Recombination between bacterial plasmids leading to the formation of plasmid multimers.
Cell. 1976 Dec;9(4 PT 2):707-16 PMID: 797459
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Gene. 1977;2(2):95-113 PMID: 344137
Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.
J Bacteriol. 1978 Jul;135(1):227-38 PMID: 97269
Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
Gene. 1978 Oct;4(2):121-36 PMID: 363519
Transfection of Enterobacteriaceae and its applications.
Microbiol Rev. 1978 Mar;42(1):194-236 PMID: 111022
Trans-complementable copy-number mutants of plasmid ColE1.
Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
Transformation and preservation of competent bacterial cells by freezing.
Methods Enzymol. 1979;68:326-31 PMID: 396437
A rapid boiling method for the preparation of bacterial plasmids.
Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome.
J Bacteriol. 1982 Apr;150(1):60-9 PMID: 6277872
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
Gene. 1981 Dec;16(1-3):237-47 PMID: 6282695
Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.
J Bacteriol. 1982 Oct;152(1):89-97 PMID: 6981642
Studies on transformation of Escherichia coli with plasmids.
J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.
J Bacteriol. 1984 Mar;157(3):809-14 PMID: 6321435

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.161.2.786-788.1985

Published

1985-02-00

Pages

786-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214956

Subset

External Links

PubMed source DOI: 10.1128/jb.161.2.786-788.1985 PMC Full Text View journal details

Analysis Services

Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

Contact

WeChat Official Account