Home LiteratureArticle Details
PMID: 6981642 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.

Journal of bacteriology ·Vol. 152 ·No. 1 ·1982-10-00 ·Pages 89-97

Fornari CS, Kaplan S

Abstract

A broad-host-range cloning vector, pUI81, was constructed in vitro from plasmids RSF1010 and pSL25 (a pBR322 derivative) and used to assay for transformation in Rhodopseudomonas sphaeroides. Washing cells with 500 mM Tris was an effective means of inducing competence for DNA uptake. Transformation frequencies as high as 10(-5) (transformants per viable cell) have been achieved by incubating Tris-treated cells with plasmid DNA, 100 mM CaCl2, and 20% polyethylene glycol 6000. Maximum frequencies were obtained when recipient cells were spread onto selective media after a 6.5-h outgrowth period in antibiotic-free medium. The structure (open circular versus closed, covalent circular), size, and concentration of plasmid DNA all significantly affected the transformation frequency. Four different plasmids, all small and suitable as cloning vectors, have been introduced by transformation into several different R. sphaeroides strains. Recombinant DNA carried on small, nonconjugative plasmids with broad host ranges can now be directly transferred to R. sphaeroides by this method.

MeSH Terms
Calcium Chloride/pharmacology DNA, Bacterial Nucleic Acid Conformation Plasmids Polyethylene Glycols/pharmacology Rhodobacter sphaeroides/genetics Transformation, Bacterial/drug effects Tromethamine/pharmacology
Chemicals
DNA, Bacterial Tromethamine Polyethylene Glycols Calcium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fornari C S
Kaplan S
References (45)
45 references, click to expand
  1. The role of a reaction center in photochemical activities of bacterial chromatophores.
    Biochim Biophys Acta. 1965 Jul 22;102(2):341-8 PMID: 5852093
  2. A requirement for sodium in the growth of Rhodopseudomonas spheroides.
    J Gen Microbiol. 1960 Jun;22:778-85 PMID: 14447230
  3. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  4. Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid.
    J Bacteriol. 1972 Jan;109(1):285-91 PMID: 4257981
  5. Membranes of photosynthetic bacteria.
    Biochim Biophys Acta. 1972 Apr 18;265(2):209-39 PMID: 4557023
  6. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  7. Genetic transformation in Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):84-7 PMID: 4630612
  8. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  9. Molecular nature of two nonconjugative plasmids carrying drug resistance genes.
    J Bacteriol. 1974 Feb;117(2):619-30 PMID: 4590480
  10. Genetic recombination in Rhodopseudomonas capsulata.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):971-3 PMID: 4522805
  11. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  12. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  13. Extrachromosomal deoxyribonucleic acid in wild-type and photosynthetically incompetent strains of Rhodopseudomonas spheroides.
    J Bacteriol. 1976 Mar;125(3):1180-7 PMID: 1082886
  14. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata.
    J Bacteriol. 1976 May;126(2):619-29 PMID: 1262313
  15. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  16. Biochemical construction of specific chimeric plasmids from ColE1 DNA and unfractionated Escherichia coli DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3838-42 PMID: 792875
  17. Studies on membrane fusion. III. The role of calcium-induced phase changes.
    Biochim Biophys Acta. 1977 Mar 17;465(3):579-98 PMID: 13835
  18. Transfer of chromosomal genes mediated by plasmid r68.45 in Rhodopseudomonas sphaeroides.
    J Bacteriol. 1977 Aug;131(2):526-32 PMID: 407213
  19. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  20. RSF1010 plasmid as a potentially useful vector in Pseudomonas species.
    J Bacteriol. 1978 Mar;133(3):1527-9 PMID: 417070
  21. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  22. Plasmid transfer and expression in Rhodopseudomonas sphaeroides.
    Arch Biochem Biophys. 1978 Apr 15;187(1):229-34 PMID: 350162
  23. Transformation of Pseudomonas putida with chromosomal DNA.
    Biochem Biophys Res Commun. 1978 May 15;82(1):281-8 PMID: 666840
  24. Viral R plasmid Rphi6P: properties of the penicillinase plasmid prophage and the supercoiled, circular encapsidated genome.
    J Bacteriol. 1978 Jul;135(1):207-14 PMID: 307547
  25. Transformation of plasmid DNA into Streptomyces at high frequency.
    Nature. 1978 Jul 27;274(5669):398-400 PMID: 672966
  26. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  27. Characterization of a site-specific restriction endonuclease from Rhodopseudomonas sphaeroides.
    J Bacteriol. 1979 May;138(2):505-9 PMID: 312287
  28. Plasmid transfer in Haemophilus influenzae.
    J Bacteriol. 1979 Aug;139(2):520-9 PMID: 313393
  29. Genetic transformation of Pseudomonas phaseolicola by R-factor DNA.
    Mol Gen Genet. 1979 Jul 2;174(1):101-3 PMID: 289900
  30. Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3348-52 PMID: 386338
  31. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  32. Effect of tris(hydroxymethyl)aminomethane on isolated sarcoplasmic reticulum vesicles.
    Arch Biochem Biophys. 1979 Nov;198(1):131-6 PMID: 159662
  33. Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata.
    J Biol Chem. 1980 Mar 25;255(6):2427-32 PMID: 7358679
  34. Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P.
    J Bacteriol. 1980 Jul;143(1):43-9 PMID: 6967484
  35. Polyethyleneglycol-induced transformation of Bacillus subtilis protoplasts by bacterial chromosomal DNA.
    Mol Gen Genet. 1980;179(3):589-94 PMID: 6777628
  36. Citrate-tris(hydroxymethyl)aminomethane-mediated release of outer membrane sections from the cell envelope of a deep-rough (heptose-deficient lipopolysaccharide) strain of Escherichia coli O8.
    J Bacteriol. 1981 Mar;145(3):1386-96 PMID: 7009584
  37. Tris(hydroxymethyl)aminomethane buffer modification of Escherichia coli outer membrane permeability.
    J Bacteriol. 1981 Mar;145(3):1397-403 PMID: 7009585
  38. Versatile cloning vector for Pseudomonas aeruginosa.
    J Bacteriol. 1981 Mar;145(3):1448-51 PMID: 6259134
  39. In vivo germinal mutation detection with "monospecific" antibody against lactate dehydrogenase-X.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7352-6 PMID: 7012839
  40. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid.
    J Bacteriol. 1981 Jun;146(3):1003-12 PMID: 6787008
  41. High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501.
    J Bacteriol. 1981 Jul;147(1):110-7 PMID: 6263862
  42. Genetic transformation.
    Annu Rev Biochem. 1981;50:41-68 PMID: 7023362
  43. Lipid-polyethylene glycol interactions: I. Induction of fusion between liposomes.
    J Membr Biol. 1981;62(1-2):65-70 PMID: 7196956
  44. Organization and differentiation of membranes of phototrophic bacteria.
    Adv Microb Physiol. 1981;22:1-92 PMID: 6460430
  45. Effects of organic cations on the gram-negative cell wall and their bactericidal activity with ethylenediaminetetra-acetate and surface active agents.
    J Gen Microbiol. 1967 Sep;48(3):391-400 PMID: 4964028
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.1.89-97.1982
Published
1982-10-00
Pages
89-97
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221378
Subset
IM
Grants
NIGMS NIH HHS · GM 7283 · United States
External Links
PubMed source DOI: 10.1128/jb.152.1.89-97.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2