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PMID: 3927151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nonproductive kappa immunoglobulin genes: recombinational abnormalities and other lesions affecting transcription, RNA processing, turnover, and translation.

Molecular and cellular biology ·Vol. 5 ·No. 7 ·1985-07-00 ·Pages 1660-75

Kelley DE, Wiedemann LM, Pittet AC, Strauss S, Nelson KJ, Davis J, Van Ness B, Perry RP

Abstract

Six nonproductive kappa immunoglobulin genes (kappa- alleles) were cloned and sequenced. The structural abnormalities discerned from sequence analysis were correlated with functional lesions at the level of transcription, RNA processing, turnover, and translation. Four kappa- alleles, three containing V kappa genes and one not, are transcribed at normal or even greater than normal rates, the defects in these genes being expressed at various posttranscriptional levels. The other two kappa- alleles, both of which lacked V genes, exhibited greatly depressed yet clearly detectable transcriptional activity. These results are consistent with a hierarchical relationship between enhancer and promoter elements in which the enhancer establishes transcriptional competence at the kappa locus and the promoter (or pseudopromoter) determines the relative level of transcriptional activity. One of the structural abnormalities discovered in this study, a large deletion which removes the entire J kappa region, also provides new insight into the mechanism of VJ and VDJ recombination.

MeSH Terms
Alleles Animals Base Sequence Cloning, Molecular Gene Expression Regulation Immunoglobulin Constant Regions/genetics Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Mice Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/metabolism Recombination, Genetic Transcription, Genetic
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains RNA, Messenger
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kelley D E
Wiedemann L M
Pittet A C
Strauss S
Nelson K J
Davis J
Van Ness B
Perry R P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.7.1660-1675.1985
Published
1985-07-00
Pages
1660-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367285
Subset
IM
Databases
GENBANK
K03461, K03462, K03463, K03464, M10115, M10116, M10117, M10118
External Links
PubMed source DOI: 10.1128/mcb.5.7.1660-1675.1985 PMC Full Text View journal details
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