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PMID: 3926945 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Extracellular K+ activity changes related to electroretinogram components. I. Amphibian (I-type) retinas.

The Journal of general physiology ·Vol. 85 ·No. 6 ·1985-06-00 ·Pages 885-909

Dick E, Miller RF

Abstract

Electroretinographic (ERG) and extracellular potassium activity measurements were carried out in superfused eyecup preparations of several amphibians. Light-evoked changes in extracellular K+ activity were characterized on the bases of depth profile analysis and latency measurements and through the application of pharmacological agents that have selective actions on the retinal network. Three different extracellular potassium modulations evoked at light onset were identified and characterized according to their phenomenological and pharmacological properties. These modulations include two separable sources of light-evoked increases in extracellular K+: (a) a proximal source that is largely post-bipolar in origin, and (b) a distal source that is primarily or exclusively of depolarizing bipolar cell origin. The pharmacological properties of the distal extracellular potassium increase closely parallel those of the b-wave. A distal light-evoked decrease in extracellular potassium appears to be associated with the slow PIII potential, based on a combination of simultaneous intracellular Müller cell recordings and extracellular ERG and potassium activity measurements before and during pharmacological isolation of the photoreceptor responses. The extracellular potassium activity increases are discussed with respect to the Müller cell theory of b-wave generation.

MeSH Terms
Ambystoma Animals Bufo marinus Electroretinography Extracellular Space/metabolism Light Membrane Potentials Microelectrodes Necturus Photic Stimulation Pimelic Acids/pharmacology Potassium/metabolism,physiology Retina/drug effects,metabolism,physiology gamma-Aminobutyric Acid/pharmacology
Chemicals
Pimelic Acids alpha-aminopimelic acid gamma-Aminobutyric Acid Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dick E
Miller R F
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59 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.85.6.885
Published
1985-06-00
Pages
885-909
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215781
Subset
IM
Grants
NEI NIH HHS · EY00844 · United States
NEI NIH HHS · EY02327 · United States
NEI NIH HHS · EY07057 · United States
External Links
PubMed source DOI: 10.1085/jgp.85.6.885 PMC Full Text View journal details
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