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PMID: 2410539 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Extracellular K+ activity changes related to electroretinogram components. II. Rabbit (E-type) retinas.

The Journal of general physiology ·Vol. 85 ·No. 6 ·1985-06-00 ·Pages 911-31

Dick E, Miller RF, Bloomfield S

Abstract

Electroretinogram (ERG) and extracellular potassium activity (K+o) measurements were carried out in isolated superfused rabbit eyecup preparations under control conditions and during the application of pharmacological agents that selectively modify the light-responsive retinal network. Light-evoked K+o changes in the rabbit (E-type) retina resemble those previously described in amphibian (I-type) retinas. Different components of the light-evoked K+o changes can be distinguished on the bases of retinal depth, V vs. log I properties, and their responses to pharmacological agents. We find two separable sources of light-evoked increases in extracellular K+: a proximal source and a distal source. The properties of the distal light-evoked K+o increase are consistent with the hypothesis that it initiates a K+-mediated current through Müller cells that is detected as the primary voltage of the electroretinographic b-wave. These experiments also support previous studies indicating that both the corneal-positive component of c-wave and the corneal-negative slow PIII potential result from K+-mediated influences on, respectively, the retinal pigment epithelium and Müller cells.

MeSH Terms
Acetylcholine/metabolism Animals Electroretinography Ethanol/pharmacology Extracellular Space/metabolism Light Membrane Potentials Microelectrodes Photic Stimulation Potassium/metabolism,physiology Rabbits Retina/drug effects,metabolism,physiology Staining and Labeling gamma-Aminobutyric Acid/pharmacology
Chemicals
Ethanol gamma-Aminobutyric Acid Acetylcholine Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dick E
Miller R F
Bloomfield S
References (35)
35 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.85.6.911
Published
1985-06-00
Pages
911-31
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215787
Subset
IM
Grants
NEI NIH HHS · EY 00844 · United States
NEI NIH HHS · EY 02327 · United States
NEI NIH HHS · EY07057-02 · United States
External Links
PubMed source DOI: 10.1085/jgp.85.6.911 PMC Full Text View journal details
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