Home LiteratureArticle Details
PMID: 3924596 Published · ppublish English Journal Article

A positive regulatory element is involved in the induction of the beta-galactosidase gene from Kluyveromyces lactis.

The EMBO journal ·Vol. 4 ·No. 3 ·1985-03-00 ·Pages 793-8

Das S, Breunig KD, Hollenberg CP

Abstract

The regulation of the LAC4 gene encoding beta-galactosidase in the yeast Kluyveromyces lactis has been studied. The expression of cloned LAC4 gene present on autonomously replicating plasmids was normally regulated by lactose or galactose as inducers. The LAC4 transcription initiation sites were mapped on two plasmids, PTY75-LAC4 and pKL2. The sites were found to be dependent on the level of gene expression and on the plasmid used. Under induced conditions, the normal cluster of initiation sites was used on both plasmids, whereas under non-induced conditions LAC4 on pKL2 showed additional sites. Deletion mapping of the 5' regulatory region of the LAC4 gene revealed a DNA element required for induction, presumably for the binding of a positive regulator.

MeSH Terms
Chromosome Deletion Enzyme Induction Galactosidases/genetics Gene Expression Regulation Genes, Regulator Plasmids Transcription, Genetic Transformation, Genetic Yeasts/genetics beta-Galactosidase/genetics
Chemicals
Galactosidases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Das S
Breunig K D
Hollenberg C P
References (24)
24 references, click to expand
  1. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  2. Catabolite repression.
    Cold Spring Harb Symp Quant Biol. 1961;26:249-56 PMID: 14468226
  3. Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment.
    Cell. 1983 Feb;32(2):371-7 PMID: 6337726
  4. Transcription of the bacterial beta-lactamase gene in Saccharomyces cerevisiae.
    Gene. 1982 Nov;20(1):1-10 PMID: 6761237
  5. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  6. Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product.
    Nucleic Acids Res. 1984 Mar 12;12(5):2327-41 PMID: 6324114
  7. Mutants in Saccharomyces lactis controlling both -glucosidase and -galactosidase activities.
    Genet Res. 1972 Feb;19(1):27-32 PMID: 5024711
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Molecular cloning and expression in E. coli of a yeast gene coding for beta-galactosidase.
    Cell. 1978 Sep;15(1):123-30 PMID: 100226
  10. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  11. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  12. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis.
    J Bacteriol. 1980 Jun;142(3):777-85 PMID: 6769910
  13. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
    Gene. 1980 Jul;10(2):157-66 PMID: 6248420
  14. Expression of a foreign eukaryotic gene in Saccharomyces cerevisiae: beta-galactosidase from Kluyveromyces lactis.
    Gene. 1980 Sep;10(4):347-56 PMID: 6253356
  15. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  16. Mutations affecting synthesis of beta-galactosidase activity in the yeast Kluyveromyces lactis.
    Genetics. 1980 Aug;95(4):877-90 PMID: 6781984
  17. Deletion mapping a eukaryotic promoter.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4461-5 PMID: 7027262
  18. Lac4 is the structural gene for beta-galactosidase in Kluyveromyces lactis.
    Genetics. 1981 Aug;98(4):729-45 PMID: 6800877
  19. Genetic regulation: yeast mutants constitutive for beta-galactosidase activity have an increased level of beta-galactosidase messenger ribonucleic acid.
    Mol Cell Biol. 1981 Nov;1(11):1048-56 PMID: 6810093
  20. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971-5 PMID: 6294669
  21. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  22. Transcriptional regulation of the Kluyveromyces lactis beta-galactosidase gene.
    Mol Cell Biol. 1981 Jul;1(7):629-34 PMID: 9279376
  23. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  24. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.
    Cell. 1983 Jan;32(1):89-98 PMID: 6337724
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-03-00
Pages
793-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554258
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2