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PMID: 3921966 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

"Exon-shuffling" maps control of antibody- and T-cell-recognition sites to the NH2-terminal domain of the class II major histocompatibility polypeptide A beta.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2940-4

Germain RN, Ashwell JD, Lechler RI, Margulies DH, Nickerson KM, Suzuki G, Tou JY

Abstract

To investigate the role of the highly polymorphic amino-terminal (beta 1) domain of the class II major histocompatibility polypeptide A beta during recognition by T cells and antibodies, "exon-shuffling" was carried out between genomic recombinant DNA clones of Ak beta and Ad beta to generate a hybrid gene containing Ak beta exons for the amino-terminal domain followed by the Ad beta exons for the remainder of the molecule. L-cell gene transfectants expressing this hybrid A beta gene in combination with Ak alpha were compared to L cells expressing wild-type Ak beta Ak alpha dimers in tests of antigen-presentation to T-cell clones and hybridomas and for staining by a panel of anti-I-Ak-specific monoclonal antibodies. These antibodies were also tested for their reactivity with a B-lymphoma transfectant expressing Ak beta in the absence of Ak alpha. The results showed no qualitative differences in either T-cell or antibody-mediated recognition of I-Ak molecules containing either the exon-shuffled or wildtype Ak beta. Together with the data involving the B cell transfectant expressing only Ak beta, these results map control of the A beta contribution to the immunologically relevant determinants of I-Ak to the highly polymorphic amino-terminal domain and indicate little, if any, contribution to allele-specific recognition by amino acid sequence variations in the remaining portions of the A beta polypeptide.

MeSH Terms
Alleles Animals Base Sequence Binding Sites DNA, Recombinant Genes, MHC Class II Histocompatibility Antigens Class II/genetics,immunology Isoantibodies/immunology L Cells/immunology Mice T-Lymphocytes/immunology
Chemicals
DNA, Recombinant Histocompatibility Antigens Class II Isoantibodies
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Germain R N
Ashwell J D
Lechler R I
Margulies D H
Nickerson K M
Suzuki G
Tou J Y
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.9.2940
Published
1985-05-00
Pages
2940-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397682
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.9.2940 PMC Full Text View journal details
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