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PMID: 3907368 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis.

The American journal of pathology ·Vol. 121 ·No. 3 ·1985-12-00 ·Pages 505-13

Yaswen P, Thompson NL, Fausto N

Abstract

Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed the CDE diet for 4 weeks. The oval cell ALKP has the same electrophoretic mobility as the enzyme from fetal rat liver and placenta. ALKPs from oval cells, parenchymal cells, and placenta all differ from the intestinal enzyme by their electrophoretic mobility, isoelectric focusing, and the patterns of amino acid inhibition of enzyme activity. Oval cells in preneoplastic livers, fetal hepatocytes, and tumor cells of a primary hepatocellular carcinoma induced by CDE feeding stained with a monoclonal antibody directed against rat placental ALKP. Hepatocytes (in normal or preneoplastic livers) and bile duct cells in normal liver did not stain with the same antibody. Placental ALKP may thus be a useful marker in tracing the origin and fate of oval cells during hepatocarcinogenesis.

MeSH Terms
Alkaline Phosphatase/analysis,antagonists & inhibitors Amino Acids/pharmacology Animals Antibodies, Monoclonal Clinical Enzyme Tests Electrophoresis, Polyacrylamide Gel Female Immunoenzyme Techniques Isoenzymes/analysis,antagonists & inhibitors Liver/enzymology Liver Neoplasms, Experimental/diagnosis Male Neuraminidase/pharmacology Placenta/enzymology Pregnancy Rats Rats, Inbred Strains Tissue Distribution
Chemicals
Amino Acids Antibodies, Monoclonal Isoenzymes Alkaline Phosphatase Neuraminidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yaswen P
Thompson N L
Fausto N
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32 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1985-12-00
Pages
505-13
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1887918
Subset
IM
Grants
NCI NIH HHS · CA 23226 · United States
External Links
PubMed source PMC Full Text View journal details
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