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PMID: 6696045 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Light- and electron-microscopic autoradiographic analysis of proliferating cells during the early stages of chemical hepatocarcinogenesis in the rat induced by feeding N-2-fluorenylacetamide in a choline-deficient diet.

The American journal of pathology ·Vol. 114 ·No. 2 ·1984-02-00 ·Pages 287-300

Sell S, Salman J

Abstract

Proliferating cells that appear after feeding the hepatocarcinogen N-2-fluorenylacetamide in a choline-deficient diet were identified by autoradiographic electron and light microscopy. Labeled cells are first seen as nondescript periductular cells 1 day after feeding carcinogen. Proliferation of duct lining cells begins at 2-3 days. For the next three weeks there is proliferation and extension of a mixture of nondescript cells and cells with a duct like appearance from the portal zone into the adjacent liver. By 3-4 weeks the entire liver lobule contains this new cell population. At 3 weeks more differentiated duct like structures are seen at the edge of the advancing new cell population. Newly appearing duct like cells differ from normal duct cells in that they contain AFP and albumin. It is concluded that the new cell population may arise from duct cells or periportal "stem" cells, or both.

MeSH Terms
2-Acetylaminofluorene/pharmacology Animals Autoradiography Cell Transformation, Neoplastic/drug effects,pathology Choline Deficiency/pathology Diet Liver/drug effects,pathology Liver Neoplasms/chemically induced,pathology Male Microscopy, Electron Rats Rats, Inbred F344 Time Factors
Chemicals
2-Acetylaminofluorene
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sell S
Salman J
References (38)
38 references, click to expand
  1. Similarities in the sequence of early histological changes induced in the liver of the rat by ethionine, 2-acetylamino-fluorene, and 3'-methyl-4-dimethylaminoazobenzene.
    Cancer Res. 1956 Feb;16(2):142-8 PMID: 13293655
  2. Autoradiography of "oval cells" appearing rapidly in the livers of rats fed N-2-fluorenylacetamide in a choline devoid diet.
    Carcinogenesis. 1981;2(1):7-14 PMID: 7273292
  3. The cellular analysis of liver carcinogenesis. V. Ultrastructural alterations within hepatocellular carcinoma induced by ethionine.
    Lab Invest. 1972 Mar;26(3):300-5 PMID: 4335732
  4. Significance of the so-called oval cell proliferation during azo-dye hepatocarcinogenesis.
    Gan. 1967 Aug;58(4):355-66 PMID: 4295056
  5. Rat alpha-fetoprotein. 8. Refinement of radioimmunoassay for detection of 1 ng rat alpha 1F.
    Immunochemistry. 1973 Jul;10(7):439-42 PMID: 4724800
  6. alpha-fetoprotein-containing cells in the early stages of liver carcinogenesis induced by 3'-methyl-4-dimethyl-aminoazobenzene and 2-acetylaminofluorene.
    Int J Cancer. 1977 Sep 15;20(3):388-93 PMID: 71280
  7. Alpha-fetoprotein: cellular origin of a biological marker in rat liver under various experimental conditions.
    Virchows Arch A Pathol Anat Histol. 1981;393(1):9-26 PMID: 6181610
  8. Quantitative histochemical and autoradiographic studies of hepatocarcinogenesis in rats fed 2-acetylaminofluorene followed by phenobarbital.
    Cancer Res. 1978 Dec;38(12):4450-7 PMID: 31235
  9. Comparison of oval cells induced in rat liver by feeding N-2-fluorenylacetamide in a choline-devoid diet and bile duct cells induced by feeding 4,4'-diaminodiphenylmethane.
    Cancer Res. 1983 Apr;43(4):1761-7 PMID: 6187438
  10. Enhancement of 2-acetylaminofluorene liver carcinogenesis in rats fed a choline-devoid diet.
    Int J Cancer. 1979 Apr 15;23(4):565-70 PMID: 220201
  11. The sequential analysis of cancer development.
    Adv Cancer Res. 1980;31:125-226 PMID: 6250325
  12. Immunofluorescent study on alpha-fetoprotein-producing cells in the early stage of 3'-methyl-4-dimethylaminoazobenzene carcinogenesis.
    Cancer Res. 1975 May;35(5):1282-7 PMID: 47267
  13. Serum alpha-fetoprotein in rats after ligation of the common bile duct: relation to ductular cell (oval cell) proliferation.
    J Pathol. 1981 Jan;133(1):61-74 PMID: 6162935
  14. Immunohistological studies on alpha1-fetoprotein and alpha1-acid glycoprotein during azo dye hepatocarcinogenesis in rats.
    Gan. 1976 Apr;67(2):253-62 PMID: 61144
  15. Progressive microscopic alterations in the livers of rats fed the hepatic carcinogens 3'-methyl-4-dimethylaminoazobenzene and 4'-fluoro-4-dimethylaminoazobenzene.
    Cancer Res. 1952 Mar;12(3):192-200 PMID: 14905443
  16. Rapid development of large numbers of alpha-fetoprotein-containing "oval" cells in the liver of rats fed N-2-fluorenylacetamide in a choline-devoid diet.
    Gan. 1981 Aug;72(4):479-87 PMID: 6171471
  17. Some factors regulating the structural integrity of the intrahepatic bile ducts with special reference to primary carcinoma of the liver and vitamin A.
    Cancer. 1954 Nov;7(6):1119-54 PMID: 13209514
  18. A comparative study of cellular organelles of Morris hepatomas.
    Cancer Res. 1972 Apr;32(4):853-67 PMID: 4335504
  19. Quantitative kinetics of development of N-2-fluorenylacetamide-induced, altered (hyperplastic) hepatocellular foci resistant to iron accumulation and of their reversion or persistence following removal of carcinogen.
    J Natl Cancer Inst. 1978 Jul;61(1):113-21 PMID: 276621
  20. Microbodies of Morris hepatomas.
    Cancer Res. 1971 Jun;31(6):763-73 PMID: 4397321
  21. Early appearance of embryonic -globulin in rat serum during carcinogenesis with 4-dimethylaminoazobenzene.
    Cancer Res. 1971 Sep;31(9):1192-4 PMID: 4107187
  22. gamma-Glutamyl transpeptidase and alpha-fetoprotein expression during alpha-naphthylisothiocyanate-induced hepatotoxicity in rats.
    Cancer Res. 1982 Dec;42(12):5133-8 PMID: 6182987
  23. ORIGIN AND FATE OF PROLIFERATED HEPATIC DUCTAL CELLS IN THE RAT: ELECTRON MICROSCOPIC AND AUTORADIOGRAPHIC STUDIES.
    Exp Mol Pathol. 1964 Jun;86:242-61 PMID: 14194323
  24. Electron microscopic studies of normal and proliferated bile ductules.
    Am J Pathol. 1961 Apr;38:393-410 PMID: 13747220
  25. Ductular cell reaction in the liver in hepatic injury.
    J Mt Sinai Hosp N Y. 1957 Sep-Oct;24(5):551-6 PMID: 13476145
  26. Cellular analysis of liver carcinogenesis. 3. Comparison of the ultrastructure of hyperplastic liver nodules and hepatocellular carcinomas induced in rat liver by 2-fluorenylacetamide.
    J Natl Cancer Inst. 1969 Jul;43(1):33-63 PMID: 4307705
  27. Expression of fibronectin and laminin in the rat liver after partial hepatectomy, during carcinogenesis, and in transplantable hepatocellular carcinomas.
    J Natl Cancer Inst. 1982 Nov;69(5):1105-14 PMID: 6290734
  28. Regression and persistence of hyperplastic hepatic nodules induced by N-2-Fluorenylacetamide and their relationship to hepatocarcinogenesis.
    Cancer Res. 1971 Jan;31(1):1-3 PMID: 5540951
  29. ETHIONINE CARCINOGENESIS.
    Adv Cancer Res. 1963;7:383-474 PMID: 14153769
  30. Cell modulation in liver pathology.
    J Histochem Cytochem. 1959 Jul;7(4):253-5 PMID: 13664975
  31. Hepatocyte proliferation and alpha 1-fetoprotein in pregnant, neonatal, and partially hepatectomized rats.
    Cancer Res. 1974 Apr;34(4):865-71 PMID: 4814999
  32. Distribution of alpha-fetoprotein- and albumin-containing cells in the livers of Fischer rats fed four cycles of N-2-fluorenylacetamide.
    Cancer Res. 1978 Sep;38(9):3107-13 PMID: 79445
  33. Heterogeneity of alpha-fetoprotein(AFP) and albumin containing cells in normal and pathological permissive states for AFP production: AFP containing cells induced in adult rats recapitulate the appearance of AFP containing hepatocytes in fetal rats.
    Oncodev Biol Med. 1980;1(2):93-105 PMID: 6169057
  34. Early elevation of alpha 1-fetoprotein in N-2-fluorenylacetamide hepatocarcinogenesis.
    Cancer Res. 1974 Oct;34(10):2489-94 PMID: 4412886
  35. Absence of stainable iron from preneoplastic and neoplastic lesions in rat liver with 8-hydroxyquinoline-induced siderosis.
    J Natl Cancer Inst. 1972 Sep;49(3):685-92 PMID: 4647491
  36. Production of fibronectin by human epithelial cells in culture.
    Cancer Res. 1979 Oct;39(10):4138-44 PMID: 383280
  37. An evaluation of cellular lineages in the pathogenesis of experimental hepatocellular carcinoma.
    Hepatology. 1982 Jan-Feb;2(1):77-86 PMID: 6172353
  38. Effect of cortisone and growth hormone upon ductular cell proliferation in liver ethionine intoxication.
    Proc Soc Exp Biol Med. 1958 Oct;99(1):24-8 PMID: 13601753
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1984-02-00
Pages
287-300
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1900337
Subset
IM
Grants
NCI NIH HHS · CA 21230 · United States
External Links
PubMed source PMC Full Text View journal details
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