Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.

Journal of bacteriology ·Vol. 164 ·No. 1 ·1985-10-00 ·Pages 470-2

Singer S, Ferone R, Walton L, Elwell L

Abstract

A strain of Escherichia coli was isolated in which dihydrofolate reductase was not detected by an enzyme assay or by competition for antibody. This strain requires methionine, glycine, a purine, and thymidine for growth in addition to the auxotrophic requirements of the parent strain. It was found to be useful as a recipient of plasmids harboring dihydrofolate reductase genes.

MeSH Terms

Escherichia coli/enzymology,genetics,growth & development Mutation Tetrahydrofolate Dehydrogenase/analysis,deficiency,genetics

Chemicals

Tetrahydrofolate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Singer S

Ferone R

Walton L

Elwell L

References (12)

12 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.164.1.470-472.1985

Published

1985-10-00

Pages

470-2

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214268

Subset

External Links

PubMed source DOI: 10.1128/jb.164.1.470-472.1985 PMC Full Text View journal details

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Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.

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