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PMID: 3891727 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of transversion mutations in Escherichia coli by N-methyl-N'-nitro-N-nitrosoguanidine is SOS dependent.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 213-20

Foster PL, Eisenstadt E

Abstract

Escherichia coli alkA mutants, which are deficient for an inducible DNA glycosylase, 3-methyladenine-DNA glycosylase II, are sensitive to mutagenesis by low doses of the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). As many as 90% of the alkA-dependent mutations induced by MNNG are also umuC+ dependent and thus are due to DNA lesions that are substrates for the mutagenic functions of the SOS response. A great number of these mutations are base substitutions at A . T sites, particularly A . T transversions. We discuss which DNA lesions may be responsible for these mutations. Our results show that the induction of 3-methyladenine-DNA glycosylase II, which occurs as part of the adaptive response to alkylating agents such as MNNG, significantly reduces the mutagenicity as well as the lethality of alkylation damage.

MeSH Terms
Alkylation DNA Glycosylases DNA Repair Escherichia coli/drug effects,genetics Histidine/genetics Methylnitronitrosoguanidine/toxicity Methyltransferases/physiology Mutation/drug effects N-Glycosyl Hydrolases/physiology O(6)-Methylguanine-DNA Methyltransferase Tryptophan/genetics
Chemicals
Methylnitronitrosoguanidine Histidine Tryptophan Methyltransferases O(6)-Methylguanine-DNA Methyltransferase DNA Glycosylases N-Glycosyl Hydrolases DNA-3-methyladenine glycosidase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Foster P L
Eisenstadt E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.213-220.1985
Published
1985-07-00
Pages
213-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219100
Subset
IM
Grants
NCI NIH HHS · CA37880 · United States
NIEHS NIH HHS · ES02021 · United States
NIEHS NIH HHS · ES03182 · United States
External Links
PubMed source DOI: 10.1128/jb.163.1.213-220.1985 PMC Full Text View journal details
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