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PMID: 3889590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1111-21

Ellis SB, Brust PF, Koutz PJ, Waters AF, Harpold MM, Gingeras TR

Abstract

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

MeSH Terms
Alcohol Oxidoreductases/genetics Ascomycota/genetics Base Sequence DNA/genetics DNA, Fungal/isolation & purification Gene Expression Regulation/drug effects Genes, Fungal Genes, Regulator Methanol/metabolism,pharmacology Pichia/enzymology,genetics Promoter Regions, Genetic Transcription, Genetic/drug effects
Chemicals
DNA, Fungal DNA Alcohol Oxidoreductases alcohol oxidase Methanol
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ellis S B
Brust P F
Koutz P J
Waters A F
Harpold M M
Gingeras T R
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1111-1121.1985
Published
1985-05-00
Pages
1111-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366829
Subset
IM
Databases
GENBANK
K03083
External Links
PubMed source DOI: 10.1128/mcb.5.5.1111-1121.1985 PMC Full Text View journal details
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