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PMID: 3885220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chicken triosephosphate isomerase complements an Escherichia coli deficiency.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 2014-8

Straus D, Gilbert W

Abstract

We present the sequence of full-length chicken triosephosphate isomerase (D-glyceraldehyde 3-phosphate ketol-isomerase, EC 5.3.1.1) mRNA based on the analysis of cDNA and genomic clones. To isolate cDNA clones encoding the enzyme, we screened a muscle cDNA library with radioactively labeled cDNA made from RNA that had been enriched by immunoselection of polysomes. We blocked the signal caused by contaminating species in the probe with cloned DNA corresponding to the contaminants. Screening a chicken genomic library with cDNA coding for triosephosphate isomerase led to the isolation of phage containing the entire gene, which we used to map the transcriptional start. When placed downstream from a hybrid trp-lac promoter, the cDNA encoding the chicken enzyme programs the synthesis of functional protein, as judged by enzymatic criteria and by complementation of an Escherichia coli mutant that is deficient in bacterial triosephosphate isomerase.

MeSH Terms
Animals Base Sequence Carbohydrate Epimerases/genetics Chickens/metabolism Cloning, Molecular Escherichia coli/enzymology,genetics Genetic Complementation Test Triose-Phosphate Isomerase/genetics
Chemicals
Carbohydrate Epimerases Triose-Phosphate Isomerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Straus D
Gilbert W
References (47)
47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.7.2014
Published
1985-04-00
Pages
2014-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397477
Subset
IM
Grants
NIGMS NIH HHS · GM09541-22 · United States
Databases
GENBANK
M11314
External Links
PubMed source DOI: 10.1073/pnas.82.7.2014 PMC Full Text View journal details
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