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PMID: 3879172 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Size of the inositol 1,4,5-trisphosphate-sensitive calcium pool in guinea-pig hepatocytes.

The Biochemical journal ·Vol. 232 ·No. 2 ·1985-12-01 ·Pages 435-8

Taylor CW, Putney JW

Abstract

Permeabilized hepatocytes accumulated 45Ca2+ into a non-mitochondrial pool when provided with ATP. 45Ca2+ efflux from this pool was revealed by removal of ATP with glucose and hexokinase or by inhibiting uptake with NaVO3. The effect of inositol 1,4,5-trisphosphate (IP3) on 45Ca2+ efflux from the pool was investigated. IP3 (5 microM) evoked a rapid increase in the rate of 45Ca2+ efflux. Kinetic analysis of the effect of IP3 indicated the existence of two distinct Ca2+ fractions within the pool; only one, accounting for about one-third of the ATP-dependent Ca2+ content of the pool, was responsive to IP3. The effect of IP3 on 45Ca2+ efflux from the non-mitochondrial pool does not require ATP, a finding that is inconsistent with a previous suggestion that this effect may be mediated by protein phosphorylation.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Calcium/pharmacology Guinea Pigs In Vitro Techniques Inositol 1,4,5-Trisphosphate Inositol Phosphates/pharmacology Kinetics Liver/cytology,drug effects,metabolism Male Sugar Phosphates/pharmacology
Chemicals
Inositol Phosphates Sugar Phosphates Inositol 1,4,5-Trisphosphate Adenosine Triphosphate Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Taylor C W
Putney J W
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20 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2320435
Published
1985-12-01
Pages
435-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152898
Subset
IM
Grants
NIADDK NIH HHS · AM-32823 · United States
External Links
PubMed source DOI: 10.1042/bj2320435 PMC Full Text View journal details
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