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PMID: 3877934 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ionic currents that generate the spontaneous diastolic depolarization in individual cardiac pacemaker cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7796-800

Shibata EF, Giles WR

Abstract

An enzymatic dispersion procedure has been developed to obtain viable, spontaneously active single myocytes from cardiac pacemaker tissue: the bullfrog (Rana catesbeiana) sinus venosus. Recordings of time- and voltage-dependent Ca2+ and K+ currents have been made by using a single suction-microelectrode technique. The results show that two time- and voltage-dependent currents interact to modulate the slope of the pacemaker potential. These are: (i) the decay of a delayed rectifier K+ current and (ii) the activation of a Ca2+ current. In addition, the data strongly suggest that cardiac pacemaker tissue does not have an inwardly rectifying background K+ current.

MeSH Terms

Animals Calcium/metabolism Diastole Heart Conduction System/physiology In Vitro Techniques Membrane Potentials Potassium/metabolism Rana catesbeiana

Chemicals

Potassium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shibata E F

Giles W R

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.22.7796

Published

1985-11-00

Pages

7796-800

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC391421

Subset

Grants

NHLBI NIH HHS · HL 27-454 · United States

NHLBI NIH HHS · HL-33662 · United States

External Links

PubMed source DOI: 10.1073/pnas.82.22.7796 PMC Full Text View journal details

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Ionic currents that generate the spontaneous diastolic depolarization in individual cardiac pacemaker cells.

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