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PMID: 3860814 Published · ppublish English Journal Article

Nucleosome core particles suppress the thermal untwisting of core DNA and adjacent linker DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 14 ·1985-07-00 ·Pages 4653-7

Morse RH, Cantor CR

Abstract

Covalently closed circular DNA is known to undergo a temperature-dependent change in helical twist. We have reconstituted nucleosome core particles onto closed circular DNA and measured the thermal untwisting of the DNA as a function of nucleosome density. The results demonstrate that the DNA associated with the nucleosome core particle does not alter its twist when the temperature is varied between 4 degrees C and 37 degrees C, and that the length of DNA prevented from thermal untwisting includes the linker as well as the core DNA.

MeSH Terms
Animals Chickens DNA/blood Electrophoresis, Polyacrylamide Gel Erythrocytes/metabolism Nucleic Acid Conformation Nucleosomes/metabolism,ultrastructure Plasmids Temperature Thermodynamics
Chemicals
Nucleosomes DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morse R H
Cantor C R
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.14.4653
Published
1985-07-00
Pages
4653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390444
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.14.4653 PMC Full Text View journal details
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