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PMID: 3858813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning and sequence analysis of cDNA for the luminescent protein aequorin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3154-8

Inouye S, Noguchi M, Sakaki Y, Takagi Y, Miyata T, Iwanaga S, Miyata T, Tsuji FI

Abstract

The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was constructed and clones carrying the cDNA for the Ca2+-dependent photoprotein were isolated by the method of colony hybridization using synthetic oligonucleotide probes. The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites. The sequence also suggested that the protein has hydrophobic regions at which the protein may interact with its functional chromophore, coelenterazine.

MeSH Terms
Aequorin/genetics Amino Acid Sequence Apoproteins/genetics Base Sequence Binding Sites Calcium/metabolism Cloning, Molecular DNA/genetics Luminescent Measurements Luminescent Proteins/genetics Protein Conformation
Chemicals
Apoproteins Luminescent Proteins Aequorin DNA Calcium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Inouye S
Noguchi M
Sakaki Y
Takagi Y
Miyata T
Iwanaga S
Miyata T
Tsuji F I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3154
Published
1985-05-00
Pages
3154-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397733
Subset
IM
Databases
GENBANK
M11394
External Links
PubMed source DOI: 10.1073/pnas.82.10.3154 PMC Full Text View journal details
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