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PMID: 383689 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The ends of Tn10 are not IS3.

Journal of bacteriology ·Vol. 139 ·No. 3 ·1979-09-00 ·Pages 1097-101

Ross DG, Grisafi P, Kleckner N, Botstein D

Abstract

By heteroduplex and hybridization analysis we showed that the inverted repetition (here called IS10) at the ends of the translocatable tetracycline resistance element Tn10 is not IS3, as had previously been reported by Ptashne and Cohen (J. Bacteriol. 122:776--781, 1975). Further analysis confirmed the homology between IS3 and the alpha beta sequence of F and demonstrated that IS10 was not present in the genomes of Salmonella typhimurium LT2 or Escherichia coli K-12.

MeSH Terms

Base Sequence Crosses, Genetic DNA, Recombinant Drug Resistance, Microbial Escherichia coli/drug effects,genetics,metabolism Lac Operon Mutation Nucleic Acid Hybridization Tetracycline/pharmacology

Chemicals

DNA, Recombinant Tetracycline

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ross D G

Grisafi P

Kleckner N

Botstein D

References (15)

15 references, click to expand

The arrangement of simian virus 40 sequences in the DNA of transformed cells.
Cell. 1976 Oct;9(2):269-87 PMID: 184963
Effects of DNA sequence non-homology on formation of bacteriophage lambda recombinants.
J Mol Biol. 1979 Jun 5;130(4):357-77 PMID: 384000
Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12.
J Bacteriol. 1978 Oct;136(1):419-22 PMID: 361699
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
Genetics. 1978 Nov;90(3):427-61 PMID: 365678
Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetitions.
Cell. 1979 Apr;16(4):721-31 PMID: 455446
Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.
Mol Gen Genet. 1973 May 9;122(3):267-77 PMID: 4577900
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
Electron microscopy of polar insertions in the lac operon of Escherichia coli.
Mol Gen Genet. 1972;119(3):207-22 PMID: 4567155
Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22.
Virology. 1972 Dec;50(3):874-82 PMID: 4565617
Packaging of an oversize transducing genome by Salmonella phage P22.
J Mol Biol. 1974 Jan 5;85(4):485-500 PMID: 4853525
Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications.
J Bacteriol. 1975 May;122(2):776-81 PMID: 1092669
Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations.
Genetics. 1975 Feb;79(2):143-74 PMID: 1093930
alphabeta sequence of F is IS31.
J Bacteriol. 1975 Aug;123(2):687-92 PMID: 1097422
Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
Proc Natl Acad Sci U S A. 1976 Jan;73(1):64-8 PMID: 1108020
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.139.3.1097-1101.1979

Published

1979-09-00

Pages

1097-101

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218067

Subset

External Links

PubMed source DOI: 10.1128/jb.139.3.1097-1101.1979 PMC Full Text View journal details

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