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PMID: 361699 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12.

Journal of bacteriology ·Vol. 136 ·No. 1 ·1978-10-00 ·Pages 419-22

Berg DE, Drummond M

Abstract

The DNA hybridization procedure of Southern has been used to search for homology between the transposable kanamycin resistance determinant Tn5 and sequences in the chromosome of Escherichia coli K-12. No homology was detected under conditions in which a segment homologous to 5% or more of the 5,300-base pair Tn5 element would have been seen.

MeSH Terms
Base Sequence Chromosomes, Bacterial DNA, Bacterial/analysis,genetics Drug Resistance, Microbial Escherichia coli/genetics,ultrastructure Genes Kanamycin/pharmacology Nucleic Acid Hybridization
Chemicals
DNA, Bacterial Kanamycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Berg D E
Drummond M
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.1.419-422.1978
Published
1978-10-00
Pages
419-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218674
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.1.419-422.1978 PMC Full Text View journal details
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