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PMID: 3822840 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Highly recurring sequence elements identified in eukaryotic DNAs by computer analysis are often homologous to regulatory sequences or protein binding sites.

Nucleic acids research ·Vol. 15 ·No. 4 ·1987-02-25 ·Pages 1835-51

Bodnar JW, Ward DC

Abstract

We have used computer assisted dot matrix and oligonucleotide frequency analyses to identify highly recurring sequence elements of 7-11 base pairs in eukaryotic genes and viral DNAs. Such elements are found much more frequently than expected, often with an average spacing of a few hundred base pairs. Furthermore, the most abundant repetitive elements observed in the ovalbumin locus, the beta-globin gene cluster, the metallothionein gene and the viral genomes of SV40, polyoma, Herpes simplex-1 and Mouse Mammary Tumor Virus were sequences shown previously to be protein binding sites or sequences important for regulating gene expression. These sequences were present in both exons and introns as well as promoter regions. These observations suggest that such sequences are often highly overrepresented within the specific gene segments with which they are associated. Computer analysis of other genetic units, including viral genomes and oncogenes, has identified a number of highly recurring sequence elements that could serve similar regulatory or protein-binding functions. A model for the role of such reiterated sequence elements in DNA organization and function is presented.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Viruses/genetics Genes Genes, Regulator Genes, Viral Protein Binding Repetitive Sequences, Nucleic Acid Retroviridae/genetics Software
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bodnar J W
Ward D C
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58 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.4.1835
Published
1987-02-25
Pages
1835-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340585
Subset
IM
Grants
NIAID NIH HHS · AI 19973 · United States
NCI NIH HHS · CA 16038 · United States
NIGMS NIH HHS · GM 32156 · United States
External Links
PubMed source DOI: 10.1093/nar/15.4.1835 PMC Full Text View journal details
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