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PMID: 3814922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Muscarinic receptors mediating suppression of the M-current in guinea-pig olfactory cortex neurones may be of the M2-subtype.

British journal of pharmacology ·Vol. 90 ·No. 1 ·1987-01-00 ·Pages 3-5

Constanti A, Sim JA

Abstract

Guinea-pig olfactory cortical neurones in vitro were voltage clamped by means of a single intracellular microelectrode technique. Hyperpolarizing voltage commands from holding potentials between -40 to -50 mV produced slow inward current relaxations reflecting deactivation of the M-current (IM). IM was reversibly suppressed by 30 microM muscarine or carbachol; this suppression was insensitive to pirenzepine (up to 300 nM) but was inhibited by gallamine (10-20 microM) or 4-diphenyl-acetoxy-N-methylpiperidine (100, 500 nM), suggesting the involvement of the M2-type muscarinic receptor.

MeSH Terms

Animals Cerebral Cortex/physiology Gallamine Triethiodide/pharmacology Guinea Pigs In Vitro Techniques Membrane Potentials/drug effects Muscarine/physiology Piperidines/pharmacology Receptors, Muscarinic/classification,drug effects

Chemicals

Piperidines Receptors, Muscarinic Muscarine 4-diphenylacetoxy-1,1-dimethylpiperidinium Gallamine Triethiodide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Constanti A

Sim J A

References (10)

10 references, click to expand

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Acetylcholine hyperpolarizes central neurones by acting on an M2 muscarinic receptor.
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M-current in voltage-clamped olfactory cortex neurones.
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Acetylcholine acts on m2-muscarinic receptors to excite rat locus coeruleus neurones.
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Article Info

Journal

British journal of pharmacology

Abbr.

Br J Pharmacol

ISSN

0007-1188

DOI

10.1111/j.1476-5381.1987.tb16818.x

Published

1987-01-00

Pages

3-5

Language

English

Region

England

NLM ID

7502536

PMCID

PMC1917286

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1987.tb16818.x PMC Full Text View journal details

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Muscarinic receptors mediating suppression of the M-current in guinea-pig olfactory cortex neurones may be of the M2-subtype.

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