How does dipyridamole elevate extracellular adenosine concentration? Predictions from a three-compartment model of adenosine formation and inactivation.

The Biochemical journal ·Vol. 237 ·No. 3 ·1986-08-01 ·Pages 845-51

Newby AC

Abstract

Steady-state mathematical models are developed according to which adenosine is formed in the cytoplasm of a group of cells, arises in the extracellular space via the symmetric nucleoside transporter and is inactivated in the adenosine forming cells and after rate-limiting transport into other cell-types. Dipyridamole increases the Km and Vmax. of the transporter symmetrically with respect to influx and efflux. Models incorporating differing degrees of compartmentation are used to predict intracellular and extracellular adenosine concentration as a function of dipyridamole concentration and adenosine formation rate. The vasodilator action of dipyridamole is explained since it is predicted to elevate interstitial fluid adenosine concentrations at all rates of adenosine formation provided that washout of the interstitial compartment is restricted.

MeSH Terms

Adenosine/antagonists & inhibitors,metabolism Adenosine Deaminase/metabolism Adenosine Kinase/metabolism Biological Transport/drug effects Cell Compartmentation/drug effects Dipyridamole/pharmacology Extracellular Space/drug effects,metabolism Models, Biological Nucleosides/antagonists & inhibitors

Chemicals

Nucleosides Dipyridamole Adenosine Kinase Adenosine Deaminase Adenosine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Newby A C

References (32)

32 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2370845

Published

1986-08-01

Pages

845-51

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1147065

Subset

External Links

PubMed source DOI: 10.1042/bj2370845 PMC Full Text View journal details

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How does dipyridamole elevate extracellular adenosine concentration? Predictions from a three-compartment model of adenosine formation and inactivation.

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