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PMID: 7159400 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Species differences in nucleoside transport. A study of uridine transport and nitrobenzylthioinosine binding by mammalian erythrocytes.

The Biochemical journal ·Vol. 208 ·No. 1 ·1982-10-15 ·Pages 83-8

Jarvis SM, Hammond JR, Paterson AR, Clanachan AS

Abstract

A kinetic study of the inward transport of uridine in erythrocytes of rabbit, human, mouse, rat and guinea-pig demonstrated that the apparent Km of this process was similar (about 0.2mM) in these cell types, but Vmax. values differed markedly. In this array of cell types, Vmax. values were proportional to the number of transport-inhibitory, high-affinity binding sites present per cell of each type. Transport of uridine or adenosine was not detected in dog erythrocytes, nor was saturable, high-affinity binding of nitrobenzylthioinosine demonstrable. These findings demonstrate that species differences in nucleoside transport capacity are attributable to differences in the cell-surface content of functional nucleoside transport sites, rather than to differences in the kinetic properties of these sites.

MeSH Terms
Animals Binding Sites Biological Transport Dogs Erythrocytes/metabolism Guinea Pigs Humans In Vitro Techniques Inosine/analogs & derivatives Kinetics Mice Rabbits Rats Species Specificity Swine Thioinosine/analogs & derivatives,blood Uridine/blood
Chemicals
Thioinosine Inosine 4-nitrobenzylthioinosine Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jarvis S M
Hammond J R
Paterson A R
Clanachan A S
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23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2080083
Published
1982-10-15
Pages
83-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153932
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2080083 PMC Full Text View journal details
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